Shigella flexneri DegP facilitates IcsA surface expression and is required for efficient intercellular spread

Georgiana Purdy, Mei Hong, Shelley M. Payne

Research output: Contribution to journalArticle

55 Citations (Scopus)

Abstract

A degP mutant of Shigella flexneri was identified in a screen for insertion mutants that invaded cultured cells but did not form wild-type plaques in monolayers. The degP mutant SM1100 invaded Henle cells at wild-type levels and induced apoptosis in macrophages but formed smaller plaques than those formed by wild-type S. flexneri in confluent monolayers of Henle and Caco-2 cells. The proportion of SM1100 bacteria with IcsA localized to the bacterial pole, a process required for actin polymerization into actin "tails," was reduced compared to results with wild-type bacteria. The reduction in proper IcsA localization may account for the reduced plaque size of the degP mutant. Although DegP is a protease, the protease activity of S. flexneri DegP was not required for IcsA localization or the formation of plaques in Henle cell monolayers. DegP was also required for efficient polar IcsA localization in E. coli expressing icsA. In addition, the growth or survival of SM1100 was compromised compared to that of the wild type at elevated temperatures and in acidic conditions.

Original languageEnglish (US)
Pages (from-to)6355-6364
Number of pages10
JournalInfection and Immunity
Volume70
Issue number11
DOIs
StatePublished - Nov 2002
Externally publishedYes

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Shigella flexneri
Actins
Bacteria
Caco-2 Cells
Polymerization
Cultured Cells
Peptide Hydrolases
Macrophages
Apoptosis
Escherichia coli
Temperature
Growth
protease S

ASJC Scopus subject areas

  • Immunology

Cite this

Shigella flexneri DegP facilitates IcsA surface expression and is required for efficient intercellular spread. / Purdy, Georgiana; Hong, Mei; Payne, Shelley M.

In: Infection and Immunity, Vol. 70, No. 11, 11.2002, p. 6355-6364.

Research output: Contribution to journalArticle

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