Sex differences in vasopressin neurons in the bed nucleus of the stria terminalis by in situ hybridization

Margaret A. Miller; Linda Vician; Donald K. Clifton; Daniel M. Dorsa

doi:10.1016/0196-9781(89)90152-6

Sex differences in vasopressin neurons in the bed nucleus of the stria terminalis by in situ hybridization

Margaret A. Miller, Linda Vician, Donald K. Clifton, Daniel M. Dorsa

Research output: Contribution to journal › Article › peer-review

118 Scopus citations

Abstract

To determine whether a sex difference exists in the biosynthetic capacity of vasopressinergic (AVP) neurons in the bed nucleus of the stria terminalis (BNST), we have used in situ hybridization and quantitative autoradiography to measure propressophysin messenger RNA levels in these cells from adult male and female rats. We have found that significantly more (p<0.01) neurons are labeled in male rats than in female rats and that these labeled cells averaged more grains/cell (p<0.05) in males than in females. Therefore, the sexual dimorphism of AVP pathways in the BNST and lateral septum recently shown by immunohistochemistry results from a sex difference in the biosynthetic capacity of these AVP neurons.

Original language	English (US)
Pages (from-to)	615-619
Number of pages	5
Journal	Peptides
Volume	10
Issue number	3
DOIs	https://doi.org/10.1016/0196-9781(89)90152-6
State	Published - 1989
Externally published	Yes

Keywords

BNST
In situ hybridization
Sex differences
Vasopressin

ASJC Scopus subject areas

Biochemistry
Physiology
Endocrinology
Cellular and Molecular Neuroscience

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10.1016/0196-9781(89)90152-6

Cite this

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title = "Sex differences in vasopressin neurons in the bed nucleus of the stria terminalis by in situ hybridization",

abstract = "To determine whether a sex difference exists in the biosynthetic capacity of vasopressinergic (AVP) neurons in the bed nucleus of the stria terminalis (BNST), we have used in situ hybridization and quantitative autoradiography to measure propressophysin messenger RNA levels in these cells from adult male and female rats. We have found that significantly more (p<0.01) neurons are labeled in male rats than in female rats and that these labeled cells averaged more grains/cell (p<0.05) in males than in females. Therefore, the sexual dimorphism of AVP pathways in the BNST and lateral septum recently shown by immunohistochemistry results from a sex difference in the biosynthetic capacity of these AVP neurons.",

keywords = "BNST, In situ hybridization, Sex differences, Vasopressin",

author = "Miller, {Margaret A.} and Linda Vician and Clifton, {Donald K.} and Dorsa, {Daniel M.}",

note = "Funding Information: We would like to thank Brian Youth, John Breininger and Paul Jones for their excellent technical assistance. This work was supported by the Veterans Administration and NIH grant NS20311.",

year = "1989",

doi = "10.1016/0196-9781(89)90152-6",

language = "English (US)",

volume = "10",

pages = "615--619",

journal = "Peptides",

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TY - JOUR

T1 - Sex differences in vasopressin neurons in the bed nucleus of the stria terminalis by in situ hybridization

AU - Miller, Margaret A.

AU - Vician, Linda

AU - Clifton, Donald K.

AU - Dorsa, Daniel M.

N1 - Funding Information: We would like to thank Brian Youth, John Breininger and Paul Jones for their excellent technical assistance. This work was supported by the Veterans Administration and NIH grant NS20311.

PY - 1989

Y1 - 1989

N2 - To determine whether a sex difference exists in the biosynthetic capacity of vasopressinergic (AVP) neurons in the bed nucleus of the stria terminalis (BNST), we have used in situ hybridization and quantitative autoradiography to measure propressophysin messenger RNA levels in these cells from adult male and female rats. We have found that significantly more (p<0.01) neurons are labeled in male rats than in female rats and that these labeled cells averaged more grains/cell (p<0.05) in males than in females. Therefore, the sexual dimorphism of AVP pathways in the BNST and lateral septum recently shown by immunohistochemistry results from a sex difference in the biosynthetic capacity of these AVP neurons.

AB - To determine whether a sex difference exists in the biosynthetic capacity of vasopressinergic (AVP) neurons in the bed nucleus of the stria terminalis (BNST), we have used in situ hybridization and quantitative autoradiography to measure propressophysin messenger RNA levels in these cells from adult male and female rats. We have found that significantly more (p<0.01) neurons are labeled in male rats than in female rats and that these labeled cells averaged more grains/cell (p<0.05) in males than in females. Therefore, the sexual dimorphism of AVP pathways in the BNST and lateral septum recently shown by immunohistochemistry results from a sex difference in the biosynthetic capacity of these AVP neurons.

KW - BNST

KW - In situ hybridization

KW - Sex differences

KW - Vasopressin

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Sex differences in vasopressin neurons in the bed nucleus of the stria terminalis by in situ hybridization

Abstract

Keywords

ASJC Scopus subject areas

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