Serum profiles of insulin-like growth factors and their binding proteins in adults with growth hormone receptor deficiency treated with insulin-like growth factor I.

P. J. Fielder, S. E. Gargosky, M. Vaccarello, K. Wilson, P. Cohen, F. Diamond, J. Guevara-Aguirre, A. L. Rosenbloom, R. G. Rosenfeld

Research output: Contribution to journalArticle

3 Scopus citations

Abstract

Six adult patients with growth hormone receptor deficiency (GHRD) (2 men, 4 women) with an identical defect in the growth hormone receptor (GHR) gene, were treated with recombinant human insulin-like growth factor I (IGF-I), 40 micrograms/kg s.c. twice daily, for 7 days. Serum concentrations of IGF peptide and IGF binding protein-3 (IGFBP-3) were measured by specific radioimmunoassays; serum IGFBPs were also measured by Western ligand blotting. The size distribution of both IGF-I and IGF-II was measured in serum following size-exclusion fast-performance liquid chromatography. IGF-I treatment resulted in a normalization of serum IGF-I levels on days 1-7 of treatment and a decrease in serum IGF-II levels. The fall in IGF-II levels and the simultaneous rise in IGF-I levels, however, resulted in an unchanged total serum IGF level. The low IGFBP-3 values did not significantly change during treatment, whereas there was a slight increase in IGFBP-2 levels. Preliminary analysis of size-fractionated sera suggested an increase in IGF-I levels in the 40 and 150 kDa regions at the expense of IGF-II levels. The results suggest that despite the failure of IGF-I treatment to increase IGFBPs significantly, serum IGFBP concentrations were sufficient to maintain normal levels of IGF-I.

Original languageEnglish (US)
Pages (from-to)40-43
Number of pages4
JournalActa paediatrica (Oslo, Norway : 1992). Supplement
Volume388
StatePublished - Mar 1 1993

ASJC Scopus subject areas

  • Pediatrics, Perinatology, and Child Health

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    Fielder, P. J., Gargosky, S. E., Vaccarello, M., Wilson, K., Cohen, P., Diamond, F., Guevara-Aguirre, J., Rosenbloom, A. L., & Rosenfeld, R. G. (1993). Serum profiles of insulin-like growth factors and their binding proteins in adults with growth hormone receptor deficiency treated with insulin-like growth factor I. Acta paediatrica (Oslo, Norway : 1992). Supplement, 388, 40-43.