Serial transplantation reveals the stem-cell-like regenerative potential of adult mouse hepatocytes

Ken Overturf, Muhsen Al-Dhalimy, Ching Nan Ou, Milton Finegold, Markus Grompe

Research output: Contribution to journalArticle

496 Scopus citations

Abstract

Previous work has shown that adult mouse hepatocytes can divide at least 18 times in vivo. To test whether this represents the upper limit of their regenerative capacity, we performed serial transplantation of hepatocytes in the fumacylacetoacetate hydrolase deficiency murine model of liver repopulation. Hepatocytes from adult donors were serially transplanted in limiting numbers six times and resulted in complete repopulation during each cycle. This corresponds to a minimal number of 69 cell doublings or a 7.3 x 1020-fold expansion. No evidence for abnormal liver function or altered hepatic architecture was found in repopulated animals. We conclude that a fraction of adult mouse hepatocytes have growth potential similar to that of hematopoietic stem cells.

Original languageEnglish (US)
Pages (from-to)1273-1280
Number of pages8
JournalAmerican Journal of Pathology
Volume151
Issue number5
StatePublished - Nov 1997

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

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