Sequence, protein expression and extracellular-regulated kinase association of the hyaladherin RHAMM (receptor for hyaluronan mediated motility) in PC12 cells

B. D. Lynn; Xinbo Li; P. A. Cattini; J. I. Nagy

doi:10.1016/S0304-3940(01)01870-5

Sequence, protein expression and extracellular-regulated kinase association of the hyaladherin RHAMM (receptor for hyaluronan mediated motility) in PC12 cells

B. D. Lynn, Xinbo Li, P. A. Cattini, J. I. Nagy

Research output: Contribution to journal › Article › peer-review

9 Scopus citations

Abstract

Differentiation of PC12 cells by nerve growth factor (NGF) or fibroblast growth factor-2 (FGF2) is dependent on signaling mediated by extracellular regulated kinase (ERK). We investigated the involvement of receptor for hyaluronan mediated motility (RHAMM) in this signaling pathway. A single RHAMM 3.2 kb transcript was detected in PC12 RNA. Reverse transcriptase-polymerase chain reaction generated a 2141 bp cDNA that had identical sequence to rat brain RHAMM and showed no evidence of alternate splicing. Several RHAMM species were identified by Western blotting. Immunohistochemistry showed RHAMM localization to the cytoskeleton, neurites and growth cones. Following stimulation of PC12 cells with NGF or FGF2 RHAMM was co-immunoprecipitated by phosphorylation-specific anti-ERK antibodies, indicating a role for RHAMM in ERK signaling in PC12 cells.

Original language	English (US)
Pages (from-to)	49-52
Number of pages	4
Journal	Neuroscience Letters
Volume	306
Issue number	1-2
DOIs	https://doi.org/10.1016/S0304-3940(01)01870-5
State	Published - Jun 22 2001
Externally published	Yes

Keywords

Cytoskeleton
Extracellular regulated kinase signaling
Growth cone
Hyaluronan
Map kinase
Receptor tyrosine kinase

ASJC Scopus subject areas

General Neuroscience

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10.1016/S0304-3940(01)01870-5

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title = "Sequence, protein expression and extracellular-regulated kinase association of the hyaladherin RHAMM (receptor for hyaluronan mediated motility) in PC12 cells",

abstract = "Differentiation of PC12 cells by nerve growth factor (NGF) or fibroblast growth factor-2 (FGF2) is dependent on signaling mediated by extracellular regulated kinase (ERK). We investigated the involvement of receptor for hyaluronan mediated motility (RHAMM) in this signaling pathway. A single RHAMM 3.2 kb transcript was detected in PC12 RNA. Reverse transcriptase-polymerase chain reaction generated a 2141 bp cDNA that had identical sequence to rat brain RHAMM and showed no evidence of alternate splicing. Several RHAMM species were identified by Western blotting. Immunohistochemistry showed RHAMM localization to the cytoskeleton, neurites and growth cones. Following stimulation of PC12 cells with NGF or FGF2 RHAMM was co-immunoprecipitated by phosphorylation-specific anti-ERK antibodies, indicating a role for RHAMM in ERK signaling in PC12 cells.",

keywords = "Cytoskeleton, Extracellular regulated kinase signaling, Growth cone, Hyaluronan, Map kinase, Receptor tyrosine kinase",

author = "Lynn, {B. D.} and Xinbo Li and Cattini, {P. A.} and Nagy, {J. I.}",

year = "2001",

month = jun,

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doi = "10.1016/S0304-3940(01)01870-5",

language = "English (US)",

volume = "306",

pages = "49--52",

journal = "Neuroscience Letters",

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T1 - Sequence, protein expression and extracellular-regulated kinase association of the hyaladherin RHAMM (receptor for hyaluronan mediated motility) in PC12 cells

AU - Lynn, B. D.

AU - Li, Xinbo

AU - Cattini, P. A.

AU - Nagy, J. I.

PY - 2001/6/22

Y1 - 2001/6/22

N2 - Differentiation of PC12 cells by nerve growth factor (NGF) or fibroblast growth factor-2 (FGF2) is dependent on signaling mediated by extracellular regulated kinase (ERK). We investigated the involvement of receptor for hyaluronan mediated motility (RHAMM) in this signaling pathway. A single RHAMM 3.2 kb transcript was detected in PC12 RNA. Reverse transcriptase-polymerase chain reaction generated a 2141 bp cDNA that had identical sequence to rat brain RHAMM and showed no evidence of alternate splicing. Several RHAMM species were identified by Western blotting. Immunohistochemistry showed RHAMM localization to the cytoskeleton, neurites and growth cones. Following stimulation of PC12 cells with NGF or FGF2 RHAMM was co-immunoprecipitated by phosphorylation-specific anti-ERK antibodies, indicating a role for RHAMM in ERK signaling in PC12 cells.

AB - Differentiation of PC12 cells by nerve growth factor (NGF) or fibroblast growth factor-2 (FGF2) is dependent on signaling mediated by extracellular regulated kinase (ERK). We investigated the involvement of receptor for hyaluronan mediated motility (RHAMM) in this signaling pathway. A single RHAMM 3.2 kb transcript was detected in PC12 RNA. Reverse transcriptase-polymerase chain reaction generated a 2141 bp cDNA that had identical sequence to rat brain RHAMM and showed no evidence of alternate splicing. Several RHAMM species were identified by Western blotting. Immunohistochemistry showed RHAMM localization to the cytoskeleton, neurites and growth cones. Following stimulation of PC12 cells with NGF or FGF2 RHAMM was co-immunoprecipitated by phosphorylation-specific anti-ERK antibodies, indicating a role for RHAMM in ERK signaling in PC12 cells.

KW - Cytoskeleton

KW - Extracellular regulated kinase signaling

KW - Growth cone

KW - Hyaluronan

KW - Map kinase

KW - Receptor tyrosine kinase

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U2 - 10.1016/S0304-3940(01)01870-5

DO - 10.1016/S0304-3940(01)01870-5

M3 - Article

C2 - 11403955

AN - SCOPUS:0035933668

SN - 0304-3940

VL - 306

SP - 49

EP - 52

JO - Neuroscience Letters

JF - Neuroscience Letters

IS - 1-2

ER -

Sequence, protein expression and extracellular-regulated kinase association of the hyaladherin RHAMM (receptor for hyaluronan mediated motility) in PC12 cells

Abstract

Keywords

ASJC Scopus subject areas

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