Sensitization of rat alveolar macrophages to enhanced TNF-α release by in vivo treatment with dexamethasone

H. Renz, A. Henke, P. Hofmann, Lawrence Wolff, A. Schmidt, J. Rüschoff, D. Gemsa

Research output: Contribution to journalArticle

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Abstract

Treatment of rats with dexamethasone rapidly induced a marked weight loss which occurred within 3 days and persisted for several weeks. The cachectic state was paralleled by increased serum levels of triglycerides, albumin, and protein and a strong reduction of blood mononuclear leukocytes. In lung sections, an increased number of mononuclear giant cells was found but no bacteria, fungi, or Pneumocystis carinii organisms. Quite strikingly, alveolar macrophages from dexamethasone-treated rats, but not from control animals, were highly sensitive to LPS and released large amounts of TNF-α ex vivo. Also under in vivo conditions, high TNF-α serum concentrations were found in dexamethasone-treated but not control rats when examined 1 1 2 hr after an intravenous LPS injection. These data, suggest that the glucocorticoid-induced cachexia of rats may be linked, at least in part, to readily inducible TNF-α release from primed macrophages.

Original languageEnglish (US)
Pages (from-to)249-257
Number of pages9
JournalCellular Immunology
Volume144
Issue number2
DOIs
StatePublished - Oct 15 1992

Fingerprint

Alveolar Macrophages
Dexamethasone
Mononuclear Leukocytes
Pneumocystis carinii
Cachexia
Giant Cells
Therapeutics
Serum
Intravenous Injections
Glucocorticoids
Weight Loss
Albumins
Triglycerides
Fungi
Macrophages
Bacteria
Lung
Proteins

ASJC Scopus subject areas

  • Cell Biology
  • Immunology

Cite this

Renz, H., Henke, A., Hofmann, P., Wolff, L., Schmidt, A., Rüschoff, J., & Gemsa, D. (1992). Sensitization of rat alveolar macrophages to enhanced TNF-α release by in vivo treatment with dexamethasone. Cellular Immunology, 144(2), 249-257. https://doi.org/10.1016/0008-8749(92)90242-H

Sensitization of rat alveolar macrophages to enhanced TNF-α release by in vivo treatment with dexamethasone. / Renz, H.; Henke, A.; Hofmann, P.; Wolff, Lawrence; Schmidt, A.; Rüschoff, J.; Gemsa, D.

In: Cellular Immunology, Vol. 144, No. 2, 15.10.1992, p. 249-257.

Research output: Contribution to journalArticle

Renz, H, Henke, A, Hofmann, P, Wolff, L, Schmidt, A, Rüschoff, J & Gemsa, D 1992, 'Sensitization of rat alveolar macrophages to enhanced TNF-α release by in vivo treatment with dexamethasone', Cellular Immunology, vol. 144, no. 2, pp. 249-257. https://doi.org/10.1016/0008-8749(92)90242-H
Renz, H. ; Henke, A. ; Hofmann, P. ; Wolff, Lawrence ; Schmidt, A. ; Rüschoff, J. ; Gemsa, D. / Sensitization of rat alveolar macrophages to enhanced TNF-α release by in vivo treatment with dexamethasone. In: Cellular Immunology. 1992 ; Vol. 144, No. 2. pp. 249-257.
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