Selection of unadapted, pathogenic SHIVs encoding newly transmitted HIV-1 envelope proteins

Gregory Q. Del Prete, Braiden Ailers, Brian Moldt, Brandon F. Keele, Jacob Estes, Anthony Rodriguez, Marissa Sampias, Kelli Oswald, Randy Fast, Charles M. Trubey, Elena Chertova, Jeremy Smedley, Celia C. Labranche, David C. Montefiori, Dennis R. Burton, George M. Shaw, Marty Markowitz, Michael Piatak, Vineet N. Kewalramani, Paul D. BieniaszJeffrey D. Lifson, Theodora Hatziioannou

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

Infection of macaques with chimeric viruses based on SIVMAC but expressing the HIV-1 envelope (Env) glycoproteins (SHIVs) remains the most powerful model for evaluating prevention and therapeutic strategies against AIDS. Unfortunately, only a few SHIVs are currently available. Furthermore, their generation has required extensive adaptation of the HIV-1 Env sequences in macaques so they may not accurately represent HIV-1 Env proteins circulating in humans, potentially limiting their translational utility. We developed a strategy for generating large numbers of SHIV constructs expressing Env proteins from newly transmitted HIV-1 strains. By inoculating macaques with cocktails of multiple SHIV variants, we selected SHIVs that can replicate and cause AIDS-like disease in immunologically intact rhesus macaques without requiring animal-to-animal passage. One of these SHIVs could be transmitted mucosally. We demonstrate the utility of the SHIVs generated by this method for evaluating neutralizing antibody administration as a protection against mucosal SHIV challenge.

Original languageEnglish (US)
Pages (from-to)412-418
Number of pages7
JournalCell Host and Microbe
Volume16
Issue number3
DOIs
StatePublished - Sep 10 2014
Externally publishedYes

Fingerprint

HIV-1
Macaca
Acquired Immunodeficiency Syndrome
Proteins
Neutralizing Antibodies
Macaca mulatta
Glycoproteins
Viruses
Infection
Therapeutics

ASJC Scopus subject areas

  • Parasitology
  • Microbiology
  • Virology

Cite this

Del Prete, G. Q., Ailers, B., Moldt, B., Keele, B. F., Estes, J., Rodriguez, A., ... Hatziioannou, T. (2014). Selection of unadapted, pathogenic SHIVs encoding newly transmitted HIV-1 envelope proteins. Cell Host and Microbe, 16(3), 412-418. https://doi.org/10.1016/j.chom.2014.08.003

Selection of unadapted, pathogenic SHIVs encoding newly transmitted HIV-1 envelope proteins. / Del Prete, Gregory Q.; Ailers, Braiden; Moldt, Brian; Keele, Brandon F.; Estes, Jacob; Rodriguez, Anthony; Sampias, Marissa; Oswald, Kelli; Fast, Randy; Trubey, Charles M.; Chertova, Elena; Smedley, Jeremy; Labranche, Celia C.; Montefiori, David C.; Burton, Dennis R.; Shaw, George M.; Markowitz, Marty; Piatak, Michael; Kewalramani, Vineet N.; Bieniasz, Paul D.; Lifson, Jeffrey D.; Hatziioannou, Theodora.

In: Cell Host and Microbe, Vol. 16, No. 3, 10.09.2014, p. 412-418.

Research output: Contribution to journalArticle

Del Prete, GQ, Ailers, B, Moldt, B, Keele, BF, Estes, J, Rodriguez, A, Sampias, M, Oswald, K, Fast, R, Trubey, CM, Chertova, E, Smedley, J, Labranche, CC, Montefiori, DC, Burton, DR, Shaw, GM, Markowitz, M, Piatak, M, Kewalramani, VN, Bieniasz, PD, Lifson, JD & Hatziioannou, T 2014, 'Selection of unadapted, pathogenic SHIVs encoding newly transmitted HIV-1 envelope proteins', Cell Host and Microbe, vol. 16, no. 3, pp. 412-418. https://doi.org/10.1016/j.chom.2014.08.003
Del Prete, Gregory Q. ; Ailers, Braiden ; Moldt, Brian ; Keele, Brandon F. ; Estes, Jacob ; Rodriguez, Anthony ; Sampias, Marissa ; Oswald, Kelli ; Fast, Randy ; Trubey, Charles M. ; Chertova, Elena ; Smedley, Jeremy ; Labranche, Celia C. ; Montefiori, David C. ; Burton, Dennis R. ; Shaw, George M. ; Markowitz, Marty ; Piatak, Michael ; Kewalramani, Vineet N. ; Bieniasz, Paul D. ; Lifson, Jeffrey D. ; Hatziioannou, Theodora. / Selection of unadapted, pathogenic SHIVs encoding newly transmitted HIV-1 envelope proteins. In: Cell Host and Microbe. 2014 ; Vol. 16, No. 3. pp. 412-418.
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