TY - JOUR
T1 - Sea urchin egg and embryo ribosomes
T2 - Differences in translational activity in a cell-free system
AU - Danilchik, Michael V.
AU - Hille, Merrill B.
N1 - Funding Information:
We would like to thank R. T. Moon for helpful discussions and assistance, D. C. Hall for technical assistance, and Drs. D. Kabat, H. F. Lodish, and G. Schubiger for critical reading of the manuscript. M.V.D. was supported by Public Health Service National Research Service Award GM 07270 from the NIH, and M.B.H. by NIH Research Grant HD 11070, University of Washington Graduate School Research Projects Fund, and Institutional Cancer Grant IN-26 from the American Cancer Society.
PY - 1981/6
Y1 - 1981/6
N2 - To determine whether ribosomes have a role in the postfertilization activation of protein synthesis in sea urchin eggs, we measured the translational activity of ribosomes isolated from unfertilized eggs and embryos of Strongylocentrotus purpuratus. Numerous previous studies have indicated few if any differences in the activity of such ribosomes. However, by using improved physiological isolation and in vitro conditions, we have found important differences in the activities of egg and embryo ribosomes. Ribosomes obtained from blastula polyribosomes were active in translating reticulocyte mRNA in a ribosome-dependent cell-free translation system, whereas ribosomes obtained from unfertilized eggs became fully active only after a characteristic, reproducible delay of up to 15 min at 26°C. The extent of this delay varied with incubation pH, but not with concentrations of K+, Mg2+, initiation factors, or mRNA. However, at incubation pH between 6.90 and 7.65, the egg ribosomes were always less active than blastula ribosomes.
AB - To determine whether ribosomes have a role in the postfertilization activation of protein synthesis in sea urchin eggs, we measured the translational activity of ribosomes isolated from unfertilized eggs and embryos of Strongylocentrotus purpuratus. Numerous previous studies have indicated few if any differences in the activity of such ribosomes. However, by using improved physiological isolation and in vitro conditions, we have found important differences in the activities of egg and embryo ribosomes. Ribosomes obtained from blastula polyribosomes were active in translating reticulocyte mRNA in a ribosome-dependent cell-free translation system, whereas ribosomes obtained from unfertilized eggs became fully active only after a characteristic, reproducible delay of up to 15 min at 26°C. The extent of this delay varied with incubation pH, but not with concentrations of K+, Mg2+, initiation factors, or mRNA. However, at incubation pH between 6.90 and 7.65, the egg ribosomes were always less active than blastula ribosomes.
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U2 - 10.1016/0012-1606(81)90397-3
DO - 10.1016/0012-1606(81)90397-3
M3 - Article
C2 - 20737867
AN - SCOPUS:0019863039
SN - 0012-1606
VL - 84
SP - 291
EP - 298
JO - Developmental Biology
JF - Developmental Biology
IS - 2
ER -