Sea urchin egg and embryo ribosomes: Differences in translational activity in a cell-free system

Michael Danilchik, Merrill B. Hille

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

To determine whether ribosomes have a role in the postfertilization activation of protein synthesis in sea urchin eggs, we measured the translational activity of ribosomes isolated from unfertilized eggs and embryos of Strongylocentrotus purpuratus. Numerous previous studies have indicated few if any differences in the activity of such ribosomes. However, by using improved physiological isolation and in vitro conditions, we have found important differences in the activities of egg and embryo ribosomes. Ribosomes obtained from blastula polyribosomes were active in translating reticulocyte mRNA in a ribosome-dependent cell-free translation system, whereas ribosomes obtained from unfertilized eggs became fully active only after a characteristic, reproducible delay of up to 15 min at 26°C. The extent of this delay varied with incubation pH, but not with concentrations of K+, Mg2+, initiation factors, or mRNA. However, at incubation pH between 6.90 and 7.65, the egg ribosomes were always less active than blastula ribosomes.

Original languageEnglish (US)
Pages (from-to)291-298
Number of pages8
JournalDevelopmental Biology
Volume84
Issue number2
DOIs
StatePublished - 1981
Externally publishedYes

Fingerprint

Sea Urchins
Cell-Free System
Ribosomes
Ovum
Embryonic Structures
Blastula
Strongylocentrotus purpuratus
Peptide Initiation Factors
Messenger RNA
Polyribosomes
Reticulocytes
Eggs

ASJC Scopus subject areas

  • Developmental Biology

Cite this

Sea urchin egg and embryo ribosomes : Differences in translational activity in a cell-free system. / Danilchik, Michael; Hille, Merrill B.

In: Developmental Biology, Vol. 84, No. 2, 1981, p. 291-298.

Research output: Contribution to journalArticle

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