Rolling blackout, a newly identified PIP2-DAG pathway lipase required for Drosophila phototransduction

Fu De Huang, Heinrich J.G. Matthies, Sean D. Speese, Mark A. Smith, Kendal Broadie

Research output: Contribution to journalArticle

49 Scopus citations

Abstract

The rolling blackout (rbo) gene encodes an integral plasma membrane lipase required for Drosophila phototransduction. Photoreceptors are enriched for the RBO protein, and temperature-sensitive rbo mutants show reversible elimination of phototransduction within minutes, demonstrating an acute requirement for the protein. The block is activity dependent, indicating that the action of RBO is use dependent. Conditional rbo mutants show activity-dependent depletion of diacylglycerol and concomitant accumulation of phosphatidylinositol phosphate and phosphatidylinositol 4,5-bisphosphate within minutes of induction, suggesting rapid downregulation of phospholipase C (PLC) activity. The RBO requirement identifies an essential regulatory step in G-protein-coupled, PLC-dependent inositol lipid signaling mediating activation of TRP and TRPL channels during phototransduction.

Original languageEnglish (US)
Pages (from-to)1070-1078
Number of pages9
JournalNature Neuroscience
Volume7
Issue number10
DOIs
StatePublished - Oct 1 2004

ASJC Scopus subject areas

  • Neuroscience(all)

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