Rolling blackout, a newly identified PIP2-DAG pathway lipase required for Drosophila phototransduction

Fu De Huang, Heinrich J G Matthies, Sean Speese, Mark A. Smith, Kendal Broadie

Research output: Contribution to journalArticle

47 Citations (Scopus)

Abstract

The rolling blackout (rbo) gene encodes an integral plasma membrane lipase required for Drosophila phototransduction. Photoreceptors are enriched for the RBO protein, and temperature-sensitive rbo mutants show reversible elimination of phototransduction within minutes, demonstrating an acute requirement for the protein. The block is activity dependent, indicating that the action of RBO is use dependent. Conditional rbo mutants show activity-dependent depletion of diacylglycerol and concomitant accumulation of phosphatidylinositol phosphate and phosphatidylinositol 4,5-bisphosphate within minutes of induction, suggesting rapid downregulation of phospholipase C (PLC) activity. The RBO requirement identifies an essential regulatory step in G-protein-coupled, PLC-dependent inositol lipid signaling mediating activation of TRP and TRPL channels during phototransduction.

Original languageEnglish (US)
Pages (from-to)1070-1078
Number of pages9
JournalNature Neuroscience
Volume7
Issue number10
DOIs
StatePublished - Oct 2004
Externally publishedYes

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Light Signal Transduction
Lipase
Drosophila
Type C Phospholipases
Diglycerides
Inositol
Phosphatidylinositols
GTP-Binding Proteins
Proteins
Down-Regulation
Cell Membrane
Lipids
Temperature
Genes

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Rolling blackout, a newly identified PIP2-DAG pathway lipase required for Drosophila phototransduction. / Huang, Fu De; Matthies, Heinrich J G; Speese, Sean; Smith, Mark A.; Broadie, Kendal.

In: Nature Neuroscience, Vol. 7, No. 10, 10.2004, p. 1070-1078.

Research output: Contribution to journalArticle

Huang, Fu De ; Matthies, Heinrich J G ; Speese, Sean ; Smith, Mark A. ; Broadie, Kendal. / Rolling blackout, a newly identified PIP2-DAG pathway lipase required for Drosophila phototransduction. In: Nature Neuroscience. 2004 ; Vol. 7, No. 10. pp. 1070-1078.
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