Robust, vaccine-induced CD8+ T lymphocyte response against an out-of-frame epitope

Nicholas J. Maness; Nancy A. Wilson; Jason S. Reed; Shari M. Piaskowski; Jonah B. Sacha; Andrew D. Walsh; Elizabeth Thoryk; Gwendolyn J. Heidecker; Michael P. Citron; Xiaoping Liang; Andrew J. Bett; Danilo R. Casimiro; David I. Watkins

doi:10.4049/jimmunol.0903118

Robust, vaccine-induced CD8⁺ T lymphocyte response against an out-of-frame epitope

Nicholas J. Maness, Nancy A. Wilson, Jason S. Reed, Shari M. Piaskowski, Jonah B. Sacha, Andrew D. Walsh, Elizabeth Thoryk, Gwendolyn J. Heidecker, Michael P. Citron, Xiaoping Liang, Andrew J. Bett, Danilo R. Casimiro, David I. Watkins

Research output: Contribution to journal › Article › peer-review

19 Scopus citations

Abstract

Rational vaccines designed to engender T cell responses require intimate knowledge of how epitopes are generated and presented. Recently, we vaccinated 8 Mamu-A*02⁺ rhesus macaques with every SIV protein except Envelope (Env). Surprisingly, one of the strongest T cell responses engendered was against the Env protein, the Mamu-A*02-restricted epitope, Env _788-795RY8. In this paper, we show that translation from an alternate reading frame of both the Rev-encoding DNA plasmid and the rAd5 vector engendered Env_788-795RY8-specific CD8⁺ T cells of greater magnitude than "normal" SIV infection. Our data demonstrate both that the pathway from vaccination to immune response is not well understood and that products of alternate reading frames may be rich and untapped sources of T cell epitopes.

Original language	English (US)
Pages (from-to)	67-72
Number of pages	6
Journal	Journal of Immunology
Volume	184
Issue number	1
DOIs	https://doi.org/10.4049/jimmunol.0903118
State	Published - Jan 1 2010
Externally published	Yes

ASJC Scopus subject areas

Immunology and Allergy
Immunology

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title = "Robust, vaccine-induced CD8+ T lymphocyte response against an out-of-frame epitope",

abstract = "Rational vaccines designed to engender T cell responses require intimate knowledge of how epitopes are generated and presented. Recently, we vaccinated 8 Mamu-A*02+ rhesus macaques with every SIV protein except Envelope (Env). Surprisingly, one of the strongest T cell responses engendered was against the Env protein, the Mamu-A*02-restricted epitope, Env 788-795RY8. In this paper, we show that translation from an alternate reading frame of both the Rev-encoding DNA plasmid and the rAd5 vector engendered Env788-795RY8-specific CD8+ T cells of greater magnitude than {"}normal{"} SIV infection. Our data demonstrate both that the pathway from vaccination to immune response is not well understood and that products of alternate reading frames may be rich and untapped sources of T cell epitopes.",

author = "Maness, {Nicholas J.} and Wilson, {Nancy A.} and Reed, {Jason S.} and Piaskowski, {Shari M.} and Sacha, {Jonah B.} and Walsh, {Andrew D.} and Elizabeth Thoryk and Heidecker, {Gwendolyn J.} and Citron, {Michael P.} and Xiaoping Liang and Bett, {Andrew J.} and Casimiro, {Danilo R.} and Watkins, {David I.}",

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AU - Maness, Nicholas J.

AU - Wilson, Nancy A.

AU - Reed, Jason S.

AU - Piaskowski, Shari M.

AU - Sacha, Jonah B.

AU - Walsh, Andrew D.

AU - Thoryk, Elizabeth

AU - Heidecker, Gwendolyn J.

AU - Citron, Michael P.

AU - Liang, Xiaoping

AU - Bett, Andrew J.

AU - Casimiro, Danilo R.

AU - Watkins, David I.

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AB - Rational vaccines designed to engender T cell responses require intimate knowledge of how epitopes are generated and presented. Recently, we vaccinated 8 Mamu-A*02+ rhesus macaques with every SIV protein except Envelope (Env). Surprisingly, one of the strongest T cell responses engendered was against the Env protein, the Mamu-A*02-restricted epitope, Env 788-795RY8. In this paper, we show that translation from an alternate reading frame of both the Rev-encoding DNA plasmid and the rAd5 vector engendered Env788-795RY8-specific CD8+ T cells of greater magnitude than "normal" SIV infection. Our data demonstrate both that the pathway from vaccination to immune response is not well understood and that products of alternate reading frames may be rich and untapped sources of T cell epitopes.

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Robust, vaccine-induced CD8⁺ T lymphocyte response against an out-of-frame epitope

Abstract

ASJC Scopus subject areas

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