RICH-1 has a BIN/Amphiphysin/Rvsp domain responsible for binding to membrane lipids and tubulation of liposomes

Ninna Richnau, Åsa Fransson, Khashayar Farsad, Pontus Aspenström

Research output: Contribution to journalArticle

30 Scopus citations

Abstract

RhoGAP interacting with CIP4 homologs-1 (RICH-1) was previously found in a yeast two-hybrid screen for proteins interacting with the SH3 domain of the Cdc42-interacting protein 4 (CIP4). RICH-1 was shown to be a RhoGAP for Cdc42 and Rac. In this study, we show that the BIN/Amphiphysin/Rvsp (BAR) domain in RICH-1 confers binding to membrane lipids, and has the potential to deform spherical liposomes into tubes. In accordance with previous findings for the BAR domains in endophilin and amphiphysin, RICH-1-induced tubes appeared striated. We propose that these striated structures are formed by oligomerization of RICH-1 through a putative coiled-coil region within the BAR domain. In support of this notion, we show that RICH-1 forms oligomers in the presence of the chemical cross-linker BS3. These results point to an involvement of RICH-1 in membrane deformation events.

Original languageEnglish (US)
Pages (from-to)1034-1042
Number of pages9
JournalBiochemical and Biophysical Research Communications
Volume320
Issue number3
DOIs
StatePublished - Jul 30 2004

Keywords

  • BAR domain
  • ER
  • Membrane deformation
  • Rho
  • RhoGAP

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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