Retinal attachment instability is diversified among mammalian melanopsins

Hisao Tsukamoto, Yoshihiro Kubo, David Farrens, Mitsumasa Koyanagi, Akihisa Terakita, Yuji Furutani

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Melanopsins play a key role in non-visual photoreception in mammals. Their close phylogenetic relationship to the photopigments in invertebrate visual cells suggests they have evolved to acquire molecular characteristics that are more suited for their non-visual functions. Here we set out to identify such characteristics by comparing the molecular properties of mammalian melanopsin to those of invertebrate melanopsin and visual pigment. Our data show that the Schiff base linking the chromophore retinal to the protein is more susceptive to spontaneous cleavage in mammalian melanopsins. We also find this stability is highly diversified between mammalian species, being particularly unstable for human melanopsin. Through mutagenesis analyses, we find that this diversified stability is mainly due to parallel amino acid substitutions in extracellular regions. We propose that the different stability of the retinal attachment in melanopsins may contribute to functional tuning of non-visual photoreception in mammals.

Original languageEnglish (US)
Pages (from-to)27176-27187
Number of pages12
JournalJournal of Biological Chemistry
Volume290
Issue number45
DOIs
StatePublished - Nov 6 2015

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Mammals
Invertebrates
Mutagenesis
Retinal Pigments
Schiff Bases
Amino Acid Substitution
Chromophores
melanopsin
Substitution reactions
Tuning
Amino Acids
Proteins

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Molecular Biology

Cite this

Retinal attachment instability is diversified among mammalian melanopsins. / Tsukamoto, Hisao; Kubo, Yoshihiro; Farrens, David; Koyanagi, Mitsumasa; Terakita, Akihisa; Furutani, Yuji.

In: Journal of Biological Chemistry, Vol. 290, No. 45, 06.11.2015, p. 27176-27187.

Research output: Contribution to journalArticle

Tsukamoto, H, Kubo, Y, Farrens, D, Koyanagi, M, Terakita, A & Furutani, Y 2015, 'Retinal attachment instability is diversified among mammalian melanopsins', Journal of Biological Chemistry, vol. 290, no. 45, pp. 27176-27187. https://doi.org/10.1074/jbc.M115.666305
Tsukamoto, Hisao ; Kubo, Yoshihiro ; Farrens, David ; Koyanagi, Mitsumasa ; Terakita, Akihisa ; Furutani, Yuji. / Retinal attachment instability is diversified among mammalian melanopsins. In: Journal of Biological Chemistry. 2015 ; Vol. 290, No. 45. pp. 27176-27187.
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