Restraint stress alters the expression of interleukin-1 and keratinocyte growth factor at the wound site

An in situ hybridization study

Ana M. Mercado, Ning Quan, David A. Padgett, John F. Sheridan, Phillip Marucha

Research output: Contribution to journalArticle

41 Citations (Scopus)

Abstract

Stress has a negative impact on wound healing. This murine study evaluated the effect of restraint stress (RST) on interleukin-1 (IL-1) and keratinocyte growth factor-1 (KGF-1) gene expression in cutaneous wounds by in situ hybridization. At day 1, RST mice had reduced frequency of IL-1β mRNA-expressing fibroblasts compared to control mice. At day 3, RST mice had reduced frequencies of IL-1β mRNA-expressing leukocytes, KGF-1 mRNA-expressing fibroblasts, and thin proliferating zones of epithelium. At day 5, RST mice had persistent leukocytic foci and reduced granulation tissue. Therefore, stress-induced alterations in cutaneous proinflammatory cytokine and growth factor expression are associated with significant histological changes in healing wounds.

Original languageEnglish (US)
Pages (from-to)74-83
Number of pages10
JournalJournal of Neuroimmunology
Volume129
Issue number1-2
DOIs
StatePublished - 2002
Externally publishedYes

Fingerprint

Fibroblast Growth Factor 7
Interleukin-1
In Situ Hybridization
Wounds and Injuries
Wound Healing
Messenger RNA
Fibroblasts
Skin
Granulation Tissue
Intercellular Signaling Peptides and Proteins
Leukocytes
Epithelium
Cytokines
Gene Expression

Keywords

  • IL-1
  • In situ hybridization
  • KGF-1
  • Mice
  • Stress
  • Wound healing

ASJC Scopus subject areas

  • Immunology
  • Clinical Neurology
  • Immunology and Allergy
  • Neurology

Cite this

Restraint stress alters the expression of interleukin-1 and keratinocyte growth factor at the wound site : An in situ hybridization study. / Mercado, Ana M.; Quan, Ning; Padgett, David A.; Sheridan, John F.; Marucha, Phillip.

In: Journal of Neuroimmunology, Vol. 129, No. 1-2, 2002, p. 74-83.

Research output: Contribution to journalArticle

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