Restraint stress alters the expression of interleukin-1 and keratinocyte growth factor at the wound site: An in situ hybridization study

Ana M. Mercado, Ning Quan, David A. Padgett, John F. Sheridan, Phillip T. Marucha

Research output: Contribution to journalArticle

43 Scopus citations


Stress has a negative impact on wound healing. This murine study evaluated the effect of restraint stress (RST) on interleukin-1 (IL-1) and keratinocyte growth factor-1 (KGF-1) gene expression in cutaneous wounds by in situ hybridization. At day 1, RST mice had reduced frequency of IL-1β mRNA-expressing fibroblasts compared to control mice. At day 3, RST mice had reduced frequencies of IL-1β mRNA-expressing leukocytes, KGF-1 mRNA-expressing fibroblasts, and thin proliferating zones of epithelium. At day 5, RST mice had persistent leukocytic foci and reduced granulation tissue. Therefore, stress-induced alterations in cutaneous proinflammatory cytokine and growth factor expression are associated with significant histological changes in healing wounds.

Original languageEnglish (US)
Pages (from-to)74-83
Number of pages10
JournalJournal of Neuroimmunology
Issue number1-2
StatePublished - Aug 23 2002



  • IL-1
  • In situ hybridization
  • KGF-1
  • Mice
  • Stress
  • Wound healing

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Neurology
  • Clinical Neurology

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