Restoration of Profiles from Slit-Scan Flow Cytometry

Richard M. Norgren, Joe Gray, Ian T. Young

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Slit-scan flow cytometry has been used to measure the distribution of fluorescent dye along mammalian chromosomes. In this procedure, the chromosomes are forced to flow lengthwise and one at a time through a thin laser beam. The resulting fluorescence profile is an approximation of the distribution of stain along the chromosome showing a dip at the centromere region. In this paper we describe a procedure, based upon Fourier analysis, to restore high spatial frequency information to the measured chromosome profile so that the centromeric dip can be precisely located. To accomplish this we estimate both the flow velocity and the laser beam profile. The procedure is applied to the restoration of profiles of fluorescent microspheres and Chinese hamster M3-1 chromosomes. Validation of the procedure is accomplished by comparing restored chromosome profiles to those obtained through high resolution fluorescence microscopy.

Original languageEnglish (US)
Pages (from-to)101-106
Number of pages6
JournalIEEE Transactions on Biomedical Engineering
VolumeBME-29
Issue number2
DOIs
StatePublished - 1982
Externally publishedYes

Fingerprint

Flow cytometry
Chromosomes
Restoration
Laser beams
Fourier analysis
Fluorescence microscopy
Microspheres
Flow velocity
Dyes
Fluorescence

ASJC Scopus subject areas

  • Biomedical Engineering

Cite this

Restoration of Profiles from Slit-Scan Flow Cytometry. / Norgren, Richard M.; Gray, Joe; Young, Ian T.

In: IEEE Transactions on Biomedical Engineering, Vol. BME-29, No. 2, 1982, p. 101-106.

Research output: Contribution to journalArticle

Norgren, Richard M. ; Gray, Joe ; Young, Ian T. / Restoration of Profiles from Slit-Scan Flow Cytometry. In: IEEE Transactions on Biomedical Engineering. 1982 ; Vol. BME-29, No. 2. pp. 101-106.
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