Abstract
Expression of the type II voltage-dependent sodium channel gene is restricted to neurons by a silencer element active in nonneuronal cells. We have cloned cDNA coding for a transcription factor (REST) that binds to this silencer element. Expression of a recombinant REST protein confers the ability to silence type II reporter genes in neuronal cell types lacking the native REST protein, whereas expression of a dominant negative form of REST in nonneuronal cells relieves silencing mediated by the native protein. REST transcripts in developing mouse embryos are detected ubiquitously outside of the nervous system. We propose that expression of the type II sodium channel gene in neurons reflects a default pathway that is blocked in nonneuronal cells by the presence of REST.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 949-957 |
| Number of pages | 9 |
| Journal | Cell |
| Volume | 80 |
| Issue number | 6 |
| DOIs | |
| State | Published - Mar 24 1995 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
- Cell Biology
- Molecular Biology
Cite this
REST : A mammalian silencer protein that restricts sodium channel gene expression to neurons. / Chong, Jayhong A.; Tapia-Ramirez, José; Kim, Sandra; Toledo-Aral, Juan J.; Zheng, Yingcong; Boutros, Michael C.; Altshuller, Yelena M.; Frohman, Michael A.; Kraner, Susan D.; Mandel, Gail.
In: Cell, Vol. 80, No. 6, 24.03.1995, p. 949-957.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - REST
T2 - A mammalian silencer protein that restricts sodium channel gene expression to neurons
AU - Chong, Jayhong A.
AU - Tapia-Ramirez, José
AU - Kim, Sandra
AU - Toledo-Aral, Juan J.
AU - Zheng, Yingcong
AU - Boutros, Michael C.
AU - Altshuller, Yelena M.
AU - Frohman, Michael A.
AU - Kraner, Susan D.
AU - Mandel, Gail
PY - 1995/3/24
Y1 - 1995/3/24
N2 - Expression of the type II voltage-dependent sodium channel gene is restricted to neurons by a silencer element active in nonneuronal cells. We have cloned cDNA coding for a transcription factor (REST) that binds to this silencer element. Expression of a recombinant REST protein confers the ability to silence type II reporter genes in neuronal cell types lacking the native REST protein, whereas expression of a dominant negative form of REST in nonneuronal cells relieves silencing mediated by the native protein. REST transcripts in developing mouse embryos are detected ubiquitously outside of the nervous system. We propose that expression of the type II sodium channel gene in neurons reflects a default pathway that is blocked in nonneuronal cells by the presence of REST.
AB - Expression of the type II voltage-dependent sodium channel gene is restricted to neurons by a silencer element active in nonneuronal cells. We have cloned cDNA coding for a transcription factor (REST) that binds to this silencer element. Expression of a recombinant REST protein confers the ability to silence type II reporter genes in neuronal cell types lacking the native REST protein, whereas expression of a dominant negative form of REST in nonneuronal cells relieves silencing mediated by the native protein. REST transcripts in developing mouse embryos are detected ubiquitously outside of the nervous system. We propose that expression of the type II sodium channel gene in neurons reflects a default pathway that is blocked in nonneuronal cells by the presence of REST.
UR - http://www.scopus.com/inward/record.url?scp=0028934541&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0028934541&partnerID=8YFLogxK
U2 - 10.1016/0092-8674(95)90298-8
DO - 10.1016/0092-8674(95)90298-8
M3 - Article
C2 - 7697725
AN - SCOPUS:0028934541
VL - 80
SP - 949
EP - 957
JO - Cell
JF - Cell
SN - 0092-8674
IS - 6
ER -