REST: A mammalian silencer protein that restricts sodium channel gene expression to neurons

Jayhong A. Chong, José Tapia-Ramirez, Sandra Kim, Juan J. Toledo-Aral, Yingcong Zheng, Michael C. Boutros, Yelena M. Altshuller, Michael A. Frohman, Susan D. Kraner, Gail Mandel

Research output: Contribution to journalArticle

841 Scopus citations

Abstract

Expression of the type II voltage-dependent sodium channel gene is restricted to neurons by a silencer element active in nonneuronal cells. We have cloned cDNA coding for a transcription factor (REST) that binds to this silencer element. Expression of a recombinant REST protein confers the ability to silence type II reporter genes in neuronal cell types lacking the native REST protein, whereas expression of a dominant negative form of REST in nonneuronal cells relieves silencing mediated by the native protein. REST transcripts in developing mouse embryos are detected ubiquitously outside of the nervous system. We propose that expression of the type II sodium channel gene in neurons reflects a default pathway that is blocked in nonneuronal cells by the presence of REST.

Original languageEnglish (US)
Pages (from-to)949-957
Number of pages9
JournalCell
Volume80
Issue number6
DOIs
StatePublished - Mar 24 1995

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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    Chong, J. A., Tapia-Ramirez, J., Kim, S., Toledo-Aral, J. J., Zheng, Y., Boutros, M. C., Altshuller, Y. M., Frohman, M. A., Kraner, S. D., & Mandel, G. (1995). REST: A mammalian silencer protein that restricts sodium channel gene expression to neurons. Cell, 80(6), 949-957. https://doi.org/10.1016/0092-8674(95)90298-8