Response of methicillin-resistant Staphylococcus aureus to amicoumacin a

Amrita Lama, Jan Pané-Farré, Tai Chon, Anna M. Wiersma, Clarissa S. Sit, John C. Vederas, Michael Hecker, Michiko Nakano

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Amicoumacin A exhibits strong antimicrobial activity against methicillin-resistant Staphylococcus aureus (MRSA), hence we sought to uncover its mechanism of action. Genome-wide transcriptome analysis of S. aureus COL in response to amicoumacin A showed alteration in transcription of genes specifying several cellular processes including cell envelope turnover, cross-membrane transport, virulence, metabolism, and general stress response. The most highly induced gene was lrgA, encoding an antiholin-like product, which is induced in cells undergoing a collapse of Δψ. Consistent with the notion that LrgA modulates murein hydrolase activity, COL grown in the presence of amicoumacin A showed reduced autolysis, which was primarily caused by lower hydrolase activity. To gain further insight into the mechanism of action of amicoumacin A, a whole genome comparison of wild-type COL and amicoumacin A-resistant mutants isolated by a serial passage method was carried out. Single point mutations generating codon substitutions were uncovered in ksgA (encoding RNA dimethyltransferase), fusA (elongation factor G), dnaG (primase), lacD (tagatose 1,6-bisphosphate aldolase), and SACOL0611 (a putative glycosyl transferase). The codon substitutions in EF-G that cause amicoumacin A resistance and fusidic acid resistance reside in separate domains and do not bring about cross resistance. Taken together, these results suggest that amicoumacin A might cause perturbation of the cell membrane and lead to energy dissipation. Decreased rates of cellular metabolism including protein synthesis and DNA replication in resistant strains might allow cells to compensate for membrane dysfunction and thus increase cell survivability.

Original languageEnglish (US)
Article numbere34037
JournalPLoS One
Volume7
Issue number3
DOIs
StatePublished - Mar 30 2012

Fingerprint

Methicillin
Methicillin-Resistant Staphylococcus aureus
hydrolases
codons
Genes
Peptide Elongation Factor G
mechanism of action
cells
fructose-bisphosphate aldolase
acid tolerance
metabolism
autolysis
genome
cross resistance
peptidoglycans
Metabolism
Codon
DNA replication
point mutation
transcriptomics

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Lama, A., Pané-Farré, J., Chon, T., Wiersma, A. M., Sit, C. S., Vederas, J. C., ... Nakano, M. (2012). Response of methicillin-resistant Staphylococcus aureus to amicoumacin a. PLoS One, 7(3), [e34037]. https://doi.org/10.1371/journal.pone.0034037

Response of methicillin-resistant Staphylococcus aureus to amicoumacin a. / Lama, Amrita; Pané-Farré, Jan; Chon, Tai; Wiersma, Anna M.; Sit, Clarissa S.; Vederas, John C.; Hecker, Michael; Nakano, Michiko.

In: PLoS One, Vol. 7, No. 3, e34037, 30.03.2012.

Research output: Contribution to journalArticle

Lama, A, Pané-Farré, J, Chon, T, Wiersma, AM, Sit, CS, Vederas, JC, Hecker, M & Nakano, M 2012, 'Response of methicillin-resistant Staphylococcus aureus to amicoumacin a', PLoS One, vol. 7, no. 3, e34037. https://doi.org/10.1371/journal.pone.0034037
Lama A, Pané-Farré J, Chon T, Wiersma AM, Sit CS, Vederas JC et al. Response of methicillin-resistant Staphylococcus aureus to amicoumacin a. PLoS One. 2012 Mar 30;7(3). e34037. https://doi.org/10.1371/journal.pone.0034037
Lama, Amrita ; Pané-Farré, Jan ; Chon, Tai ; Wiersma, Anna M. ; Sit, Clarissa S. ; Vederas, John C. ; Hecker, Michael ; Nakano, Michiko. / Response of methicillin-resistant Staphylococcus aureus to amicoumacin a. In: PLoS One. 2012 ; Vol. 7, No. 3.
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