Requirement for microtubules in new membrane formation during cytokinesis of Xenopus embryos

Michael Danilchik, W. C. Funk, E. E. Brown, K. Larkin

Research output: Contribution to journalArticle

100 Citations (Scopus)

Abstract

In cleaving Xenopus eggs, exposure to nocodazole or cold shock prevents the addition of new plasma membrane to the cleavage plane and causes furrows to recede, suggesting a specific role for microtubules in cytokinesis. Whole- mount confocal immunocytochemistry reveals a ring of radially arranged, acetylated microtubule bundles at the base of all advancing cleavage furrows, from the first cleavage through the midblastula stage. We hypothesize that this novel microtubular structure is involved in transporting maternal stores of membrane in the subcortex to a site of membrane addition near the leading edge of the furrow.

Original languageEnglish (US)
Pages (from-to)47-60
Number of pages14
JournalDevelopmental Biology
Volume194
Issue number1
DOIs
StatePublished - Feb 1 1998

Fingerprint

Cytokinesis
Xenopus
Microtubules
Embryonic Structures
Nocodazole
Membranes
Eggs
Shock
Immunohistochemistry
Mothers
Cell Membrane

Keywords

  • Cell division
  • Cleavage furrow
  • Confocal microscopy
  • Cytokinesis
  • Egg
  • Embryo
  • Membrane
  • Microtubule
  • Midbody
  • Midzone
  • Xenopu s

ASJC Scopus subject areas

  • Developmental Biology

Cite this

Requirement for microtubules in new membrane formation during cytokinesis of Xenopus embryos. / Danilchik, Michael; Funk, W. C.; Brown, E. E.; Larkin, K.

In: Developmental Biology, Vol. 194, No. 1, 01.02.1998, p. 47-60.

Research output: Contribution to journalArticle

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