Replacements in the exposed loop of the T15 antibody VH CDR2 affect carrier recognition of PC-containing pathogens

McKay Brown, Gregory D. Wiens, Thomas O'Hare, Mary Stenzel-Poore, Marvin Rittenberg

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

A panel of mutant antibodies of the phosphocholine (PC)-binding antibody, T15, was tested for binding to PC-protein, Streptococcus pneumoniae, Trichinella spiralis and Ascaris suum. Relative to wildtype T15, all the mutant antibodies showed differential recognition of the panel of PC- associated antigens. These mutant antibodies contain amino acid replacements in the CDR2 region of the heavy chain variable region, indicating the importance of CDR2 in recognition of carrier determinants. A model of T15 is shown that illustrates the strategic placement of mutations that could allow interaction with determinants associated with PC. A direct implication of this finding is that the T15 antibody combining site accommodates structures larger than phosphocholine and that recognition of associated carrier determinants could be a significant force in shaping the immune response to PC-containing pathogens.

Original languageEnglish (US)
Pages (from-to)205-211
Number of pages7
JournalMolecular Immunology
Volume36
Issue number3
DOIs
StatePublished - Feb 1999

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Phosphorylcholine
Antibodies
Ascaris suum
Trichinella spiralis
Streptococcus pneumoniae
Binding Sites
Antigens
Amino Acids
Mutation
Proteins

Keywords

  • Antibody CDR
  • Mutation
  • Phosphocholine
  • T15 antibody

ASJC Scopus subject areas

  • Molecular Biology
  • Immunology

Cite this

Replacements in the exposed loop of the T15 antibody VH CDR2 affect carrier recognition of PC-containing pathogens. / Brown, McKay; Wiens, Gregory D.; O'Hare, Thomas; Stenzel-Poore, Mary; Rittenberg, Marvin.

In: Molecular Immunology, Vol. 36, No. 3, 02.1999, p. 205-211.

Research output: Contribution to journalArticle

Brown, McKay ; Wiens, Gregory D. ; O'Hare, Thomas ; Stenzel-Poore, Mary ; Rittenberg, Marvin. / Replacements in the exposed loop of the T15 antibody VH CDR2 affect carrier recognition of PC-containing pathogens. In: Molecular Immunology. 1999 ; Vol. 36, No. 3. pp. 205-211.
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