Repair response of human fibroblasts to bleomycin damage

Myra M. Hurt, Arthur L. Beaudet, Robb Moses

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

The ability of human fibroblasts to repair the specific types of DNA damage caused by bleomycin (BLM) was examined in whole-cell experiments. The method utilized for analysis was alkaline sucrose-gradient centrifugation of DNA. The results of these studies show that a repair pathway exists for the damage produced in DNA by bleomycin. DNA from BLM-treated cells shows a decrease in molecular weight, caused by chemical or enzymatic incision at sites of drug action. If the drug is removed, the DNA rapidly returns to high molecular weight, demonstrating reformation of damaged DNA. This repair response to BLM-damage was also confirmed in fibroblasts isolated from patients with putative DNA-repair defects. We observed that the response (to BLM) of cells from patients with Fanconi anemia was altered in that the fall in molecular weight of DNA from treated cells was not as great as that observed in other cell strains after drug treatment.

Original languageEnglish (US)
Pages (from-to)181-189
Number of pages9
JournalMutation Research DNA Repair Reports
Volume112
Issue number3
DOIs
StatePublished - 1983
Externally publishedYes

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Bleomycin
Fibroblasts
DNA
Molecular Weight
Pharmaceutical Preparations
Fanconi Anemia
Centrifugation
DNA Repair
DNA Damage
Sucrose

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Repair response of human fibroblasts to bleomycin damage. / Hurt, Myra M.; Beaudet, Arthur L.; Moses, Robb.

In: Mutation Research DNA Repair Reports, Vol. 112, No. 3, 1983, p. 181-189.

Research output: Contribution to journalArticle

Hurt, Myra M. ; Beaudet, Arthur L. ; Moses, Robb. / Repair response of human fibroblasts to bleomycin damage. In: Mutation Research DNA Repair Reports. 1983 ; Vol. 112, No. 3. pp. 181-189.
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