Renal function of gene-targeted mice lacking both SGK1 and SGK3

Florian Grahammer, Ferruh Artunc, Diana Sandulache, Rexhep Rexhepaj, Björn Friedrich, Teut Risler, James (Jim) McCormick, Kevin Dawson, Jian Wang, David Pearce, Peer Wulff, Dietmar Kuhl, Florian Lang

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

Serum- and glucocorticoid-inducible kinase (SGK) 1 and SGK3 share the ability to upregulate several ion channels, including the epithelial Na + channel. Whereas SGK1 is under genomic control of mineralocorticoids and glucocorticoids, SGK3 is constitutively expressed. The SKG1-knockout (sgk1-/-) mouse is seemingly normal when it is fed a standard diet, but its ability to retain NaCl is impaired when it is fed a salt-deficient diet. In the SGK3-knockout (sgk3-/-) mouse fed standard and salt-deficient diets, hair growth is strikingly delayed but NaCl excretion is normal. Thus the possibility was considered that SGK1 and SGK3 could mutually replace each other, thus preventing severe NaCl loss in sgk1 -/- and sgk3-/- mice. We crossed SGK1- and SGK3-knockout mice and compared renal electrolyte excretion of the double mutants (sgk1 -/-/sgk3-/-) with that of their wildtype littermates (sgk1+/+/sgk3+/+). Similar to sgk3-/- mice, the sgk1-/-/sgk3-/- mice display delayed hair growth. Blood pressure was slightly, but significantly (P <0.03), lower in sgk1 -/-/sgk3-/- (102 ± 4 mmHg) than in sgk1 +/+/sgk3+/+ (114 ± 3 mmHg) mice, a difference that was maintained in mice fed low- and high-salt diets. Plasma aldosterone concentrations were significantly (P <0.01) higher in sgk1 -/-/sgk3-/- than in sgk1+/+sgk3+/+ mice fed control (511 ± 143 vs. 143 ± 32 pg/ml) and low-salt (1,325 ± 199 vs. 362 ± 145 pg/ml) diets. During salt depletion, absolute and fractional excretions of Na+ were significantly (P <0.01) higher in sgk1-/-/sgk3-/- (1.2 ± 0.2 μmol/24 h g body wt, 0.12 ± 0.03%) than in sgk1+/+/ sgk3+/+ (0.4 ± 0.1 μmol/24 h g body wt, 0.04 ± 0.01%) mice. The sgk1-/-/sgk3-/- mice share the delayed hair growth with sgk3-/- mice and the modestly impaired renal salt retention with sgk1-/- mice. Additional lack of the isoform kinase does not substantially compound the phenotype for either property.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Regulatory Integrative and Comparative Physiology
Volume290
Issue number4
DOIs
StatePublished - Apr 2006
Externally publishedYes

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Knockout Mice
Salts
Kidney
Genes
Diet
Hair
Glucocorticoids
Growth
Epithelial Sodium Channels
Mineralocorticoids
Aldosterone
Ion Channels
Electrolytes
Protein Isoforms
Phosphotransferases
Up-Regulation
Blood Pressure
Phenotype

Keywords

  • Aldosterone
  • Blood pressure
  • Epithelial sodium channel
  • Kidney
  • Mineralocorticoids

ASJC Scopus subject areas

  • Physiology

Cite this

Renal function of gene-targeted mice lacking both SGK1 and SGK3. / Grahammer, Florian; Artunc, Ferruh; Sandulache, Diana; Rexhepaj, Rexhep; Friedrich, Björn; Risler, Teut; McCormick, James (Jim); Dawson, Kevin; Wang, Jian; Pearce, David; Wulff, Peer; Kuhl, Dietmar; Lang, Florian.

In: American Journal of Physiology - Regulatory Integrative and Comparative Physiology, Vol. 290, No. 4, 04.2006.

Research output: Contribution to journalArticle

Grahammer, F, Artunc, F, Sandulache, D, Rexhepaj, R, Friedrich, B, Risler, T, McCormick, JJ, Dawson, K, Wang, J, Pearce, D, Wulff, P, Kuhl, D & Lang, F 2006, 'Renal function of gene-targeted mice lacking both SGK1 and SGK3', American Journal of Physiology - Regulatory Integrative and Comparative Physiology, vol. 290, no. 4. https://doi.org/10.1152/ajpregu.00484.2005
Grahammer, Florian ; Artunc, Ferruh ; Sandulache, Diana ; Rexhepaj, Rexhep ; Friedrich, Björn ; Risler, Teut ; McCormick, James (Jim) ; Dawson, Kevin ; Wang, Jian ; Pearce, David ; Wulff, Peer ; Kuhl, Dietmar ; Lang, Florian. / Renal function of gene-targeted mice lacking both SGK1 and SGK3. In: American Journal of Physiology - Regulatory Integrative and Comparative Physiology. 2006 ; Vol. 290, No. 4.
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AU - Risler, Teut

AU - McCormick, James (Jim)

AU - Dawson, Kevin

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KW - Aldosterone

KW - Blood pressure

KW - Epithelial sodium channel

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