Renal denervation in male rats with heart failure improves ventricular sympathetic nerve innervation and function

Maximilian I. Pinkham, Michael T. Loftus, Satya Amirapu, Sarah Jane Guild, Gina Quill, William Woodward, Beth Habecker, Carolyn J. Barrett

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Heart failure is characterized by the loss of sympathetic innervation to the ventricles, contributing to impaired cardiac function and arrhythmogenesis. We hypothesized that renal denervation (RDx) would reverse this loss. Male Wistar rats underwent myocardial infarction (MI) or sham surgery and progressed into heart failure for 4 wk before receiving bilateral RDx or sham RDx. After additional 3 wk, left ventricular (LV) function was assessed, and ventricular sympathetic nerve fiber density was determined via histology. Post-MI heart failure rats displayed significant reductions in ventricular sympathetic innervation and tissue norepinephrine content (nerve fiber density in the LV of MI+sham RDx hearts was 0.31 ± 0.05% vs. 1.00 ± 0.10% in sham MI+sham RDx group, P < 0.05), and RDx significantly increased ventricular sympathetic innervation (0.76 ± 0.14%, P < 0.05) and tissue norepinephrine content. MI was associated with an increase in fibrosis of the noninfarcted ventricular myocardium, which was attenuated by RDx. RDx improved LV ejection fraction and end-systolic and -diastolic areas when compared with pre-RDx levels. This is the first study to show an interaction between renal nerve activity and cardiac sympathetic nerve innervation in heart failure. Our findings show denervating the renal nerves improves cardiac sympathetic innervation and function in the post-MI failing heart.

Original languageEnglish (US)
Pages (from-to)R368-R379
JournalAmerican Journal of Physiology - Regulatory Integrative and Comparative Physiology
Volume312
Issue number3
DOIs
StatePublished - Mar 10 2017

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Denervation
Heart Failure
Myocardial Infarction
Kidney
Nerve Fibers
Norepinephrine
Adrenergic Fibers
Left Ventricular Function
Stroke Volume
Wistar Rats
Myocardium
Histology
Fibrosis

Keywords

  • Cardiac innervation
  • Heart failure
  • Renal nerve
  • Sympathetic nerve activity

ASJC Scopus subject areas

  • Physiology
  • Physiology (medical)

Cite this

Renal denervation in male rats with heart failure improves ventricular sympathetic nerve innervation and function. / Pinkham, Maximilian I.; Loftus, Michael T.; Amirapu, Satya; Guild, Sarah Jane; Quill, Gina; Woodward, William; Habecker, Beth; Barrett, Carolyn J.

In: American Journal of Physiology - Regulatory Integrative and Comparative Physiology, Vol. 312, No. 3, 10.03.2017, p. R368-R379.

Research output: Contribution to journalArticle

Pinkham, Maximilian I. ; Loftus, Michael T. ; Amirapu, Satya ; Guild, Sarah Jane ; Quill, Gina ; Woodward, William ; Habecker, Beth ; Barrett, Carolyn J. / Renal denervation in male rats with heart failure improves ventricular sympathetic nerve innervation and function. In: American Journal of Physiology - Regulatory Integrative and Comparative Physiology. 2017 ; Vol. 312, No. 3. pp. R368-R379.
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