Reinnervation of dopamine-denervated striatum by substantia nigra transplants: Immunohistochemical and electrophysiological correlates

I. Strömberg, Steven Johnson, B. Hoffer, L. Olson

Research output: Contribution to journalArticle

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Abstract

This study evaluated whether or not fetal substantia nigra tissue, grafted to striatum previously lesioned with 6-hydroxydopamine, provides functional dopaminergic reinnervation of striatum. Falck-Hillarp histochemistry and immunofluorescent staining for tyrosine hydroxylase demonstrated extensive networks of nerve fibers which extended 1-1.5 mm from the nigral grafts into striatal tissue. Multibarrel micropipettes were used to record neurons electrophysiologically and test neuronal responses to phencyclidine which was applied locally by pressure microejection. "Distal" neurons, defined as those striatal neurons more than 2.0 mm from the nigral graft, fired at an average spontaneous rate of 13.4 spikes/s and were relatively insensitive to the effects of locally applied phencyclidine. However, "proximal" neurons, defined as those neurons less than 1.0 mm from nigral grafts, fired at a significantly lower average rate of 4.9 spikes/s, and were significantly more sensitive than distal neurons to the effects of phencyclidine. These results suggest that fetal substantia nigra grafts can provide functionally significant reinnervation of striatum previously lesioned with 6-hydroxydopamine.

Original languageEnglish (US)
Pages (from-to)981-990
Number of pages10
JournalNeuroscience
Volume14
Issue number4
DOIs
StatePublished - 1985
Externally publishedYes

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Substantia Nigra
Dopamine
Transplants
Neurons
Phencyclidine
Corpus Striatum
Oxidopamine
Tyrosine 3-Monooxygenase
Nerve Fibers
Staining and Labeling
Pressure

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Reinnervation of dopamine-denervated striatum by substantia nigra transplants : Immunohistochemical and electrophysiological correlates. / Strömberg, I.; Johnson, Steven; Hoffer, B.; Olson, L.

In: Neuroscience, Vol. 14, No. 4, 1985, p. 981-990.

Research output: Contribution to journalArticle

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