Regulation of corepressor function by nuclear NADH

Qinghong Zhang, David W. Piston, Richard Goodman

Research output: Contribution to journalArticle

433 Citations (Scopus)

Abstract

The corepressor CtBP (carboxyl-terminal binding protein) is involved in transcriptional pathways important for development, cell cycle regulation, and transformation. We demonstrate that CtBP binding to cellular and viral transcriptional repressors is regulated by the nicotinamide adenine dinucleotides NAD+ and NADH, with NADH being two to three orders of magnitude more effective. Levels of free nuclear nicotinamide adenine dinucleotides, determined using two-photon microscopy, correspond to the levels required for half-maximal CtBP binding and are considerably lower than those previously reported. Agents capable of increasing NADH levels stimulate CtBP binding to its partners in vivo and potentiate CtBP-mediated repression. We propose that this ability to detect changes in nuclear NAD+/NADH ratio allows CtBP to serve as a redox sensor for transcription.

Original languageEnglish (US)
Pages (from-to)1895-1897
Number of pages3
JournalScience
Volume295
Issue number5561
DOIs
StatePublished - Mar 8 2002

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Co-Repressor Proteins
NAD
Carrier Proteins
Protein Binding
Photons
Oxidation-Reduction
Microscopy
Cell Cycle

ASJC Scopus subject areas

  • General

Cite this

Regulation of corepressor function by nuclear NADH. / Zhang, Qinghong; Piston, David W.; Goodman, Richard.

In: Science, Vol. 295, No. 5561, 08.03.2002, p. 1895-1897.

Research output: Contribution to journalArticle

Zhang, Qinghong ; Piston, David W. ; Goodman, Richard. / Regulation of corepressor function by nuclear NADH. In: Science. 2002 ; Vol. 295, No. 5561. pp. 1895-1897.
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