TY - JOUR
T1 - Reduction of human blood O2 affinity using dihydroxyacetone, phosphate, and pyruvate
AU - Kerr, H. H.
AU - Pantely, G. A.
AU - Metcalfe, J.
AU - Welch, J. E.
PY - 1979
Y1 - 1979
N2 - Human blood oxygen affinity (BOA) was measured after blood from 6 normal donors was incubated with 4 concentrations of dihydroxyacetone (0.022, 0.044, 0.088, and 0.175 M) plus equimolar disodium phosphate and pyruvate (sodium salt) (0.013, 0.025, 0.05 and 0.1 M) in solutions labeled DDP x 1, DDP x 2, DDP x 4, and DDP x 8, respectively. Blood P50 rose (BOA was reduced) from a control value of 26.0 ± 0.4 Torr (mean ± SD) to 29.4 ± 0.6, 30.6 ± 0.4, 31.9 ± 0.15, and 33.3 ± 1.4 Torr after 2 hr of incubation at 37° C with solutions DDP x 1, DDP x 2, DDP x 4, and DDP x 8, respectively. P50 changes at 2 hr were 75% complete within 30 min. During these incubations, erythrocyte 2,3-diphosphoglycerate (2,3-DPG) concentration rose from 0.76 ± 0.09 mol/mol Hb (control) to 1.09 ± 0.17, 1.14 ± 0.10, 1.33 ± 0.15, and 1.45 ± 0.25 mol/mol Hb with increasing solution concentration. BOA is decreased by an increase in erythrocyte 2,3-DPG. Reduced BOA may improve oxygen delivery to ischemic tissues.
AB - Human blood oxygen affinity (BOA) was measured after blood from 6 normal donors was incubated with 4 concentrations of dihydroxyacetone (0.022, 0.044, 0.088, and 0.175 M) plus equimolar disodium phosphate and pyruvate (sodium salt) (0.013, 0.025, 0.05 and 0.1 M) in solutions labeled DDP x 1, DDP x 2, DDP x 4, and DDP x 8, respectively. Blood P50 rose (BOA was reduced) from a control value of 26.0 ± 0.4 Torr (mean ± SD) to 29.4 ± 0.6, 30.6 ± 0.4, 31.9 ± 0.15, and 33.3 ± 1.4 Torr after 2 hr of incubation at 37° C with solutions DDP x 1, DDP x 2, DDP x 4, and DDP x 8, respectively. P50 changes at 2 hr were 75% complete within 30 min. During these incubations, erythrocyte 2,3-diphosphoglycerate (2,3-DPG) concentration rose from 0.76 ± 0.09 mol/mol Hb (control) to 1.09 ± 0.17, 1.14 ± 0.10, 1.33 ± 0.15, and 1.45 ± 0.25 mol/mol Hb with increasing solution concentration. BOA is decreased by an increase in erythrocyte 2,3-DPG. Reduced BOA may improve oxygen delivery to ischemic tissues.
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U2 - 10.1152/jappl.1979.47.3.478
DO - 10.1152/jappl.1979.47.3.478
M3 - Article
C2 - 118143
AN - SCOPUS:0018730247
SN - 0161-7567
VL - 47
SP - 478
EP - 481
JO - Journal of Applied Physiology Respiratory Environmental and Exercise Physiology
JF - Journal of Applied Physiology Respiratory Environmental and Exercise Physiology
IS - 3
ER -