Reduction of human blood O2 affinity using dihydroxyacetone, phosphate, and pyruvate

H. H. Kerr, G. A. Pantely, J. Metcalfe, J. E. Welch

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    1 Scopus citations

    Abstract

    Human blood oxygen affinity (BOA) was measured after blood from 6 normal donors was incubated with 4 concentrations of dihydroxyacetone (0.022, 0.044, 0.088, and 0.175 M) plus equimolar disodium phosphate and pyruvate (sodium salt) (0.013, 0.025, 0.05 and 0.1 M) in solutions labeled DDP x 1, DDP x 2, DDP x 4, and DDP x 8, respectively. Blood P50 rose (BOA was reduced) from a control value of 26.0 ± 0.4 Torr (mean ± SD) to 29.4 ± 0.6, 30.6 ± 0.4, 31.9 ± 0.15, and 33.3 ± 1.4 Torr after 2 hr of incubation at 37° C with solutions DDP x 1, DDP x 2, DDP x 4, and DDP x 8, respectively. P50 changes at 2 hr were 75% complete within 30 min. During these incubations, erythrocyte 2,3-diphosphoglycerate (2,3-DPG) concentration rose from 0.76 ± 0.09 mol/mol Hb (control) to 1.09 ± 0.17, 1.14 ± 0.10, 1.33 ± 0.15, and 1.45 ± 0.25 mol/mol Hb with increasing solution concentration. BOA is decreased by an increase in erythrocyte 2,3-DPG. Reduced BOA may improve oxygen delivery to ischemic tissues.

    Original languageEnglish (US)
    Pages (from-to)478-481
    Number of pages4
    JournalJournal of Applied Physiology Respiratory Environmental and Exercise Physiology
    Volume47
    Issue number3
    StatePublished - Dec 1 1979

    ASJC Scopus subject areas

    • Physiology
    • Endocrinology

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