Red blood cell PUFAs reflect the phospholipid PUFA composition of major organs

Jenifer I. Fenton, Eric A. Gurzell, Emily A. Davidson, William Harris

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Numerous clinical trials examining the use of omega-3 long chain polyunsaturated fatty acids (n-3 LCPUFAs) on various health outcomes have been conducted, and fish oil remains one of the most widely used nutritional supplements. More recently, studies have begun to utilize the omega-3 index, defined as the sum of EPA+DHA in red blood cells (RBCs), as both a biomarker of n-3 LCPUFA exposure and a potential risk factor for coronary heart disease (CHD). Considerably less research evaluates whether RBC phospholipid fatty acids reflect the phospholipid fatty acid composition of other tissues across increasing intakes of n-3 LCPUFAs. We fed mice diets containing increasing amounts of EPA+DHA, equivalent to current recommendations by the American Heart Association on a percent of energy basis, and analyzed the phospholipid fatty acid composition of various tissues in relation to RBCs. We observed that RBCs, heart, muscle, spleen, lung, and adipose tissues all respond to dietary supplementation with EPA+DHA with increasing n-3 LCPUFA and decreasing n-6 LCPUFA levels. Furthermore, the n-3 LCPUFA profiles of all measured tissues had strong (r>0.7) and significant (p<0.001) correlations to RBCs. Interestingly, we also observed changes in saturated fatty acid (SFA) and monounsaturated fatty acid (MUFA) levels across various tissues in response to increased EPA+DHA intakes despite there being no change in dietary SFA and MUFA. Specifically, there were increases in RBC SFA and spleen MUFA and decreases in heart MUFA. These demonstrate that the RBC, including the omega-3 index, may serve as a marker for the relative levels of n-3 and n-6 LCPUFAs in phospholipids of certain tissues.

Original languageEnglish (US)
Pages (from-to)12-23
Number of pages12
JournalProstaglandins Leukotrienes and Essential Fatty Acids
Volume112
DOIs
StatePublished - Sep 1 2016
Externally publishedYes

Fingerprint

Phospholipids
Blood
Erythrocytes
Cells
Monounsaturated Fatty Acids
Fatty Acids
Tissue
Chemical analysis
Spleen
Fish Oils
Omega-3 Fatty Acids
Biomarkers
Nutrition
Dietary Supplements
Coronary Disease
Muscle
Adipose Tissue
Myocardium
Health
Clinical Trials

Keywords

  • Adipose
  • Biomarker
  • Fish oil
  • Heart
  • Lung
  • Muscle
  • Omega-3 index
  • Phospholipid
  • PUFA
  • RBC

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Cell Biology

Cite this

Red blood cell PUFAs reflect the phospholipid PUFA composition of major organs. / Fenton, Jenifer I.; Gurzell, Eric A.; Davidson, Emily A.; Harris, William.

In: Prostaglandins Leukotrienes and Essential Fatty Acids, Vol. 112, 01.09.2016, p. 12-23.

Research output: Contribution to journalArticle

Fenton, Jenifer I. ; Gurzell, Eric A. ; Davidson, Emily A. ; Harris, William. / Red blood cell PUFAs reflect the phospholipid PUFA composition of major organs. In: Prostaglandins Leukotrienes and Essential Fatty Acids. 2016 ; Vol. 112. pp. 12-23.
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