Recruitment of calcium-permeable AMPA receptors during synaptic potentiation is regulated by CaM-kinase I

Eric S. Guire, Michael C. Oh, Thomas Soderling, Victor A. Derkach

    Research output: Contribution to journalArticle

    103 Citations (Scopus)

    Abstract

    Ca2+-permeable AMPA receptors (CP-AMPARs) at central glutamatergic synapses are of special interest because of their unique biophysical and signaling properties that contribute to synaptic plasticity and their roles in multiple neuropathologies. However, intracellular signaling pathways that recruit synaptic CP-AMPARs are unknown, and involvement of CP-AMPARs in hippocampal region CA1 synaptic plasticity is controversial. Here, we report that intracellular infusion of active CaM-kinase I (CaMKI) into cultured hippocampal neurons enhances miniature EPSC amplitude because of recruitment of CP-AMPARs, likely from an extrasynaptic pool. The ability of CaMKI, which regulates the actin cytoskeleton, to recruit synaptic CP-AMPARs was blocked by inhibiting actin polymerization with latrunculin A. CaMK regulation of CP-AMPARs was also confirmed in hippocampal slices. CA1 long-term potentiation (LTP) after theta bursts, but not high-frequency tetani, produced a rapid, transient expression of synaptic CP-AMPARs that facilitated LTP. This component of TBS LTP was blocked by inhibition of CaM-kinase kinase (CaMKK), the upstream activator of CaMKI. Our calculations show that adding CP-AMPARs numbering

    Original languageEnglish (US)
    Pages (from-to)6000-6009
    Number of pages10
    JournalJournal of Neuroscience
    Volume28
    Issue number23
    DOIs
    StatePublished - Jun 4 2008

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    Calcium-Calmodulin-Dependent Protein Kinase Type 1
    AMPA Receptors
    Calcium
    Long-Term Potentiation
    Neuronal Plasticity
    Calcium-Calmodulin-Dependent Protein Kinase Kinase
    Phosphotransferases
    Hippocampal CA1 Region
    Tetanus
    Actin Cytoskeleton
    Polymerization
    Synapses
    Actins

    Keywords

    • AMPA receptor
    • Calcium
    • CaM-kinase
    • Hippocampus
    • LTP
    • Synaptic plasticity

    ASJC Scopus subject areas

    • Neuroscience(all)
    • Medicine(all)

    Cite this

    Recruitment of calcium-permeable AMPA receptors during synaptic potentiation is regulated by CaM-kinase I. / Guire, Eric S.; Oh, Michael C.; Soderling, Thomas; Derkach, Victor A.

    In: Journal of Neuroscience, Vol. 28, No. 23, 04.06.2008, p. 6000-6009.

    Research output: Contribution to journalArticle

    Guire, Eric S. ; Oh, Michael C. ; Soderling, Thomas ; Derkach, Victor A. / Recruitment of calcium-permeable AMPA receptors during synaptic potentiation is regulated by CaM-kinase I. In: Journal of Neuroscience. 2008 ; Vol. 28, No. 23. pp. 6000-6009.
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