Recombinant thrombopoietin induces rapid protein tyrosine phosphorylation of Janus kinase 2 and Shc in human blood platelets

Y. Miyakawa, A. Oda, Brian Druker, T. Kato, H. Miyazaki, M. Handa, Y. Ikeda

Research output: Contribution to journalArticle

129 Citations (Scopus)

Abstract

A cDNA for the thrombopoietin has been cloned by several groups. The recombinant thrombopoietin has been reported to stimulate the megakaryocytopoiesis and thrombopoiesis. Little is known regarding the molecular basis of its effects. To elucidate the molecular mechanism involved in signal transduction, we have investigated the effects of thrombopoietin on platelet tyrosine phosphorylation. We report here that thrombopoietin induced time- and dose-dependent tyrosine phosphorylation of several proteins including Janus kinase 2 (Jak2) and a 52-kD protein, Shc, in human blood platelets. Both Jak2 and Shc were tyrosine phosphorylated within 15 seconds after stimulation. The tyrosine phosphorylation of Jak2 was accompanied by increased kinase activity, whereas Shc tyrosine phosphorylation induced its association with a 25-kD protein, Grb2. Thus, our data suggest that Jak2, Shc, and Grb2 may be involved in signal transduction after ligand binding to c-mpl in human platelets.

Original languageEnglish (US)
Pages (from-to)23-27
Number of pages5
JournalBlood
Volume86
Issue number1
StatePublished - 1995
Externally publishedYes

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Janus Kinase 2
Thrombopoietin
Phosphorylation
Platelets
Tyrosine
Blood
Blood Platelets
Thrombopoiesis
Signal transduction
Proteins
Signal Transduction
Phosphotransferases
Complementary DNA
Association reactions
Ligands

ASJC Scopus subject areas

  • Hematology

Cite this

Miyakawa, Y., Oda, A., Druker, B., Kato, T., Miyazaki, H., Handa, M., & Ikeda, Y. (1995). Recombinant thrombopoietin induces rapid protein tyrosine phosphorylation of Janus kinase 2 and Shc in human blood platelets. Blood, 86(1), 23-27.

Recombinant thrombopoietin induces rapid protein tyrosine phosphorylation of Janus kinase 2 and Shc in human blood platelets. / Miyakawa, Y.; Oda, A.; Druker, Brian; Kato, T.; Miyazaki, H.; Handa, M.; Ikeda, Y.

In: Blood, Vol. 86, No. 1, 1995, p. 23-27.

Research output: Contribution to journalArticle

Miyakawa, Y, Oda, A, Druker, B, Kato, T, Miyazaki, H, Handa, M & Ikeda, Y 1995, 'Recombinant thrombopoietin induces rapid protein tyrosine phosphorylation of Janus kinase 2 and Shc in human blood platelets', Blood, vol. 86, no. 1, pp. 23-27.
Miyakawa, Y. ; Oda, A. ; Druker, Brian ; Kato, T. ; Miyazaki, H. ; Handa, M. ; Ikeda, Y. / Recombinant thrombopoietin induces rapid protein tyrosine phosphorylation of Janus kinase 2 and Shc in human blood platelets. In: Blood. 1995 ; Vol. 86, No. 1. pp. 23-27.
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