Reciprocal changes in fructose-6-phosphate,2-kinase and fructose-2,6-bisphosphatase activity in response to glucagon and epinephrine

Carolyn (Sue) Richards, Motoko Yokoyama, Eisuke Furuya, Kosaku Uyeda

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

The effect of hormones on the enzymes responsible for the synthesis (fructose-6-P,2-kinase) and degradation (fructose-2,6-Pase) of fructose-2,6-P2 was examined in isolated rat hepatocytes. Glucagon (10-11 M), epinephrine (10-5 M), or calcium (2.4 mM) and A23187 (10-5 M) administration to hepatocytes produced simultaneous activation of fructose-2,6-Pase and inactivation of fructose-6-P,2-kinase within 2 minutes. The effect of epinephrine on these two enzymes was dependent on the presence of Ca++. These results suggest that the level of fructose-2,6-P2 is controlled by recriprocal changes in fructose-2,6-Pase and fructose-6-P,2-kinase activities.

Original languageEnglish (US)
Pages (from-to)1073-1079
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume104
Issue number3
DOIs
StatePublished - Feb 11 1982
Externally publishedYes

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Phosphofructokinase-2
Fructose
Glucagon
Epinephrine
Hepatocytes
Calcimycin
Enzymes
Rats
Chemical activation
Hormones
Calcium
Degradation

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Reciprocal changes in fructose-6-phosphate,2-kinase and fructose-2,6-bisphosphatase activity in response to glucagon and epinephrine. / Richards, Carolyn (Sue); Yokoyama, Motoko; Furuya, Eisuke; Uyeda, Kosaku.

In: Biochemical and Biophysical Research Communications, Vol. 104, No. 3, 11.02.1982, p. 1073-1079.

Research output: Contribution to journalArticle

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