Rat RIβ isoform of type I regulatory subunit of cyclic adenosine monophosphate‐dependent protein kinase: cDNA sequence analysis, mRNA tissue specificity, and rat/mouse difference in expression in testis

John S. Massa, Robert E. Fellows, Richard A. Maurer

Research output: Contribution to journalArticle

8 Scopus citations

Abstract

A rat complementary DNA (cDNA) for the RIβ isoform of type I cyclic adenosine monophosphate (cAMP)‐dependent protein kinase regulatory subunit was cloned and sequenced and was found to contain the entire protein coding and 3′‐untranslated regions, with a single (ATTAAA) poly‐adenylation site. The largest open reading frame was preceded by a short outof‐phase open reading frame, which is not seen in the corresponding mouse RIβ cDNA due to a single base substitution. The rat RIβ cDNA clone was 2,374 bases long and detected a rat mRNA of approximately 2.8 kilobases. Rat RIβ mRNA was abundant in adult rat brain and testis but was undetectable in other rat tissues. The rat RIβ cDNA also detected RIβ mRNA in mouse brain, but not mouse testis, from 10‐week‐old BALB/c or 10‐ and 6‐week‐old Swiss Webster mice. Thus, despite a 96% nucleotide identity in the coding region of RIβ in rat vs. mouse, there are at least two differences in these closely related species. First, there is a short open reading frame, which precedes the coding region in the rat but not the mouse. Second, unlike the mouse testis, the rat testis contains abundant levels of RIβ mRNA.

Original languageEnglish (US)
Pages (from-to)129-133
Number of pages5
JournalMolecular Reproduction and Development
Volume26
Issue number2
DOIs
StatePublished - Jun 1990

Keywords

  • Kinase isoforms
  • Protein kinase A
  • cAMP

ASJC Scopus subject areas

  • Genetics
  • Developmental Biology
  • Cell Biology

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