TY - JOUR
T1 - Rat RIβ isoform of type I regulatory subunit of cyclic adenosine monophosphate‐dependent protein kinase
T2 - cDNA sequence analysis, mRNA tissue specificity, and rat/mouse difference in expression in testis
AU - Massa, John S.
AU - Fellows, Robert E.
AU - Maurer, Richard A.
PY - 1990/6
Y1 - 1990/6
N2 - A rat complementary DNA (cDNA) for the RIβ isoform of type I cyclic adenosine monophosphate (cAMP)‐dependent protein kinase regulatory subunit was cloned and sequenced and was found to contain the entire protein coding and 3′‐untranslated regions, with a single (ATTAAA) poly‐adenylation site. The largest open reading frame was preceded by a short outof‐phase open reading frame, which is not seen in the corresponding mouse RIβ cDNA due to a single base substitution. The rat RIβ cDNA clone was 2,374 bases long and detected a rat mRNA of approximately 2.8 kilobases. Rat RIβ mRNA was abundant in adult rat brain and testis but was undetectable in other rat tissues. The rat RIβ cDNA also detected RIβ mRNA in mouse brain, but not mouse testis, from 10‐week‐old BALB/c or 10‐ and 6‐week‐old Swiss Webster mice. Thus, despite a 96% nucleotide identity in the coding region of RIβ in rat vs. mouse, there are at least two differences in these closely related species. First, there is a short open reading frame, which precedes the coding region in the rat but not the mouse. Second, unlike the mouse testis, the rat testis contains abundant levels of RIβ mRNA.
AB - A rat complementary DNA (cDNA) for the RIβ isoform of type I cyclic adenosine monophosphate (cAMP)‐dependent protein kinase regulatory subunit was cloned and sequenced and was found to contain the entire protein coding and 3′‐untranslated regions, with a single (ATTAAA) poly‐adenylation site. The largest open reading frame was preceded by a short outof‐phase open reading frame, which is not seen in the corresponding mouse RIβ cDNA due to a single base substitution. The rat RIβ cDNA clone was 2,374 bases long and detected a rat mRNA of approximately 2.8 kilobases. Rat RIβ mRNA was abundant in adult rat brain and testis but was undetectable in other rat tissues. The rat RIβ cDNA also detected RIβ mRNA in mouse brain, but not mouse testis, from 10‐week‐old BALB/c or 10‐ and 6‐week‐old Swiss Webster mice. Thus, despite a 96% nucleotide identity in the coding region of RIβ in rat vs. mouse, there are at least two differences in these closely related species. First, there is a short open reading frame, which precedes the coding region in the rat but not the mouse. Second, unlike the mouse testis, the rat testis contains abundant levels of RIβ mRNA.
KW - Kinase isoforms
KW - Protein kinase A
KW - cAMP
UR - http://www.scopus.com/inward/record.url?scp=0025367962&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0025367962&partnerID=8YFLogxK
U2 - 10.1002/mrd.1080260206
DO - 10.1002/mrd.1080260206
M3 - Article
C2 - 2372396
AN - SCOPUS:0025367962
SN - 1040-452X
VL - 26
SP - 129
EP - 133
JO - Molecular Reproduction and Development
JF - Molecular Reproduction and Development
IS - 2
ER -