Abstract
DNA sequencing of several independent rat IGF-I cDNA clones has revealed three different 5′-untranslated region sequences which contain multiple, upstream, in-frame initiation codons. Use of these codons could generate N-terminal heterogeneity in IGF-I precursor proteins. One of these 5′-untranslated region sequences contains a 40-bp segment which is an inverted repeat of a region in the common 3′-untranslated region. The ends of the IGF-I mRNA corresponding to this cDNA could form a stable duplex structure. Such a complex could prevent ribosomal access to the AUG codons preceeding the coding region for the pre-pro-IGF-I peptide, suggesting the possibility of translational regulation of this form of IGF-I mRNA. The 3′-untranslated region inverted repeat sequence also is present in human and mouse IGF-I cDNA's, and, intriguingly, is more highly conserved than the rest of the 3′-untranslated region.
Original language | English (US) |
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Pages (from-to) | 1154-1159 |
Number of pages | 6 |
Journal | Biochemical and Biophysical Research Communications |
Volume | 146 |
Issue number | 3 |
DOIs | |
State | Published - Aug 14 1987 |
Externally published | Yes |
ASJC Scopus subject areas
- Biophysics
- Biochemistry
- Molecular Biology
- Cell Biology