Rat IGF-I cDNA's contain multiple 5′-untranslated regions

Charles Roberts, Stephen R. Lasky, William L. Lowe, Derek LeRoith

Research output: Contribution to journalArticle

62 Citations (Scopus)

Abstract

DNA sequencing of several independent rat IGF-I cDNA clones has revealed three different 5′-untranslated region sequences which contain multiple, upstream, in-frame initiation codons. Use of these codons could generate N-terminal heterogeneity in IGF-I precursor proteins. One of these 5′-untranslated region sequences contains a 40-bp segment which is an inverted repeat of a region in the common 3′-untranslated region. The ends of the IGF-I mRNA corresponding to this cDNA could form a stable duplex structure. Such a complex could prevent ribosomal access to the AUG codons preceeding the coding region for the pre-pro-IGF-I peptide, suggesting the possibility of translational regulation of this form of IGF-I mRNA. The 3′-untranslated region inverted repeat sequence also is present in human and mouse IGF-I cDNA's, and, intriguingly, is more highly conserved than the rest of the 3′-untranslated region.

Original languageEnglish (US)
Pages (from-to)1154-1159
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume146
Issue number3
DOIs
StatePublished - Aug 14 1987
Externally publishedYes

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5' Untranslated Regions
Insulin-Like Growth Factor I
Rats
Complementary DNA
3' Untranslated Regions
Codon
Inverted Repeat Sequences
Messenger RNA
Protein Precursors
Initiator Codon
DNA Sequence Analysis
Clone Cells
Peptides
DNA

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Rat IGF-I cDNA's contain multiple 5′-untranslated regions. / Roberts, Charles; Lasky, Stephen R.; Lowe, William L.; LeRoith, Derek.

In: Biochemical and Biophysical Research Communications, Vol. 146, No. 3, 14.08.1987, p. 1154-1159.

Research output: Contribution to journalArticle

Roberts, Charles ; Lasky, Stephen R. ; Lowe, William L. ; LeRoith, Derek. / Rat IGF-I cDNA's contain multiple 5′-untranslated regions. In: Biochemical and Biophysical Research Communications. 1987 ; Vol. 146, No. 3. pp. 1154-1159.
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