Rat cellular retinol-binding protein: cDNA sequence and rapid retinol-dependent accumulation of mRNA

D. R. Sherman, R. S. Lloyd, F. Chytil

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Cellular retinol-binding protein (CRBP) may be an important mediator of vitamin A action. We report here the identification of a cDNA clone corresponding to the rat CRBP gene. The cDNA is 695 nucleotides long, with an open reading frame corresponding to a protein of 134 amino acids. The deduced amino acid sequence is identical with that of rat CRBP. The nucleotide sequence shows 90.5% similarity with the human CRBP cDNA sequence. Genomic DNA analysis indicates that CRBP is present in one, or at most two, copies within the rat genome. Analysis of mRNA reveals a single species in every tissue tested and suggests that the isolated cDNA is full-length. Finally, when retinol-deficient rats are fed retinyl acetate for 4 hr, about 4-fold accumulation of CRBP-specific mRNA is observed in the lungs. This rapid effect suggests that the micronutrient retinol may directly influence the expression of its specific intracellular binding protein.

Original languageEnglish (US)
Pages (from-to)3209-3213
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Issue number10
StatePublished - Jan 1 1987


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