Rat cellular retinol-binding protein: cDNA sequence and rapid retinol-dependent accumulation of mRNA

D. R. Sherman, Robert (Stephen) Lloyd, F. Chytil

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

Cellular retinol-binding protein (CRBP) may be an important mediator of vitamin A action. We report here the identification of a cDNA clone corresponding to the rat CRBP gene. The cDNA is 695 nucleotides long, with an open reading frame corresponding to a protein of 134 amino acids. The deduced amino acid sequence is identical with that of rat CRBP. The nucleotide sequence shows 90.5% similarity with the human CRBP cDNA sequence. Genomic DNA analysis indicates that CRBP is present in one, or at most two, copies within the rat genome. Analysis of mRNA reveals a single species in every tissue tested and suggests that the isolated cDNA is full-length. Finally, when retinol-deficient rats are fed retinyl acetate for 4 hr, about 4-fold accumulation of CRBP-specific mRNA is observed in the lungs. This rapid effect suggests that the micronutrient retinol may directly influence the expression of its specific intracellular binding protein.

Original languageEnglish (US)
Pages (from-to)3209-3213
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume84
Issue number10
StatePublished - 1987
Externally publishedYes

Fingerprint

Cellular Retinol-Binding Proteins
Vitamin A
Complementary DNA
Messenger RNA
Micronutrients
Open Reading Frames
Amino Acid Sequence
Carrier Proteins
Nucleotides
Clone Cells
Genome
Amino Acids
Lung
DNA
Genes

ASJC Scopus subject areas

  • General
  • Genetics

Cite this

@article{7859fb068d0847e58ab20de8030ba8c9,
title = "Rat cellular retinol-binding protein: cDNA sequence and rapid retinol-dependent accumulation of mRNA",
abstract = "Cellular retinol-binding protein (CRBP) may be an important mediator of vitamin A action. We report here the identification of a cDNA clone corresponding to the rat CRBP gene. The cDNA is 695 nucleotides long, with an open reading frame corresponding to a protein of 134 amino acids. The deduced amino acid sequence is identical with that of rat CRBP. The nucleotide sequence shows 90.5{\%} similarity with the human CRBP cDNA sequence. Genomic DNA analysis indicates that CRBP is present in one, or at most two, copies within the rat genome. Analysis of mRNA reveals a single species in every tissue tested and suggests that the isolated cDNA is full-length. Finally, when retinol-deficient rats are fed retinyl acetate for 4 hr, about 4-fold accumulation of CRBP-specific mRNA is observed in the lungs. This rapid effect suggests that the micronutrient retinol may directly influence the expression of its specific intracellular binding protein.",
author = "Sherman, {D. R.} and Lloyd, {Robert (Stephen)} and F. Chytil",
year = "1987",
language = "English (US)",
volume = "84",
pages = "3209--3213",
journal = "Proceedings of the National Academy of Sciences of the United States of America",
issn = "0027-8424",
number = "10",

}

TY - JOUR

T1 - Rat cellular retinol-binding protein

T2 - cDNA sequence and rapid retinol-dependent accumulation of mRNA

AU - Sherman, D. R.

AU - Lloyd, Robert (Stephen)

AU - Chytil, F.

PY - 1987

Y1 - 1987

N2 - Cellular retinol-binding protein (CRBP) may be an important mediator of vitamin A action. We report here the identification of a cDNA clone corresponding to the rat CRBP gene. The cDNA is 695 nucleotides long, with an open reading frame corresponding to a protein of 134 amino acids. The deduced amino acid sequence is identical with that of rat CRBP. The nucleotide sequence shows 90.5% similarity with the human CRBP cDNA sequence. Genomic DNA analysis indicates that CRBP is present in one, or at most two, copies within the rat genome. Analysis of mRNA reveals a single species in every tissue tested and suggests that the isolated cDNA is full-length. Finally, when retinol-deficient rats are fed retinyl acetate for 4 hr, about 4-fold accumulation of CRBP-specific mRNA is observed in the lungs. This rapid effect suggests that the micronutrient retinol may directly influence the expression of its specific intracellular binding protein.

AB - Cellular retinol-binding protein (CRBP) may be an important mediator of vitamin A action. We report here the identification of a cDNA clone corresponding to the rat CRBP gene. The cDNA is 695 nucleotides long, with an open reading frame corresponding to a protein of 134 amino acids. The deduced amino acid sequence is identical with that of rat CRBP. The nucleotide sequence shows 90.5% similarity with the human CRBP cDNA sequence. Genomic DNA analysis indicates that CRBP is present in one, or at most two, copies within the rat genome. Analysis of mRNA reveals a single species in every tissue tested and suggests that the isolated cDNA is full-length. Finally, when retinol-deficient rats are fed retinyl acetate for 4 hr, about 4-fold accumulation of CRBP-specific mRNA is observed in the lungs. This rapid effect suggests that the micronutrient retinol may directly influence the expression of its specific intracellular binding protein.

UR - http://www.scopus.com/inward/record.url?scp=0023217954&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0023217954&partnerID=8YFLogxK

M3 - Article

C2 - 3472205

AN - SCOPUS:0023217954

VL - 84

SP - 3209

EP - 3213

JO - Proceedings of the National Academy of Sciences of the United States of America

JF - Proceedings of the National Academy of Sciences of the United States of America

SN - 0027-8424

IS - 10

ER -