Rapid membrane effects of steroids in neuroblastoma cells

Effects of estrogen on mitogen activated protein kinase signalling cascade and c-fos immediate early gene transcription

Jyoti J. Watters, Jean S. Campbell, Matthew J. Cunningham, Edwin G. Krebs, Daniel Dorsa

Research output: Contribution to journalArticle

429 Citations (Scopus)

Abstract

Rapid effects of steroid hormones have been observed in neuronal cells for many years. We show here, that in the human neuroblastoma cell line SK- N-SH, the membrane impermeable conjugated 17β-estradiol (E2BSA) activates mitogen activated protein kinase kinase (MAPKK or MEK) and induces the phosphorylation and activation of both ERK-1 and ERK-2 (mitogen activated protein kinase or MAPK). Additionally, E2BSA induces the transcription of a reporter gene construct driven by the promoter of the mouse c-fos proto- oncogene. The effects of this membrane impermeable estrogen on c-fos transcription are not inhibited by the estrogen receptor antagonists Tamoxifen or ICI 182,780, further excluding the involvement of the intracellular estrogen receptor. This is also illustrated by the observation that E2BSA does not activate estrogen response element (ERE) mediated transcription. This is the first report of rapid membrane effects of 17β- estradiol on growth factor related signalling pathways in neuronal cells, and indicates a potential mechanism by which 17β-estradiol might affect the expression of genes whose promoters do not contain EREs but are responsive to factors acting through other response elements such as AP-1 and SRE sites.

Original languageEnglish (US)
Pages (from-to)4030-4033
Number of pages4
JournalEndocrinology
Volume138
Issue number9
DOIs
StatePublished - 1997
Externally publishedYes

Fingerprint

Genetic Transcription
fos Genes
Immediate-Early Genes
Mitogen-Activated Protein Kinase Kinases
Mitogen-Activated Protein Kinases
Neuroblastoma
Estradiol
Estrogens
Steroids
Response Elements
Membranes
Mitogen-Activated Protein Kinase 1
Transcription Factor AP-1
Tamoxifen
Reporter Genes
Estrogen Receptors
Intercellular Signaling Peptides and Proteins
Phosphorylation
Hormones
Gene Expression

ASJC Scopus subject areas

  • Endocrinology
  • Endocrinology, Diabetes and Metabolism

Cite this

Rapid membrane effects of steroids in neuroblastoma cells : Effects of estrogen on mitogen activated protein kinase signalling cascade and c-fos immediate early gene transcription. / Watters, Jyoti J.; Campbell, Jean S.; Cunningham, Matthew J.; Krebs, Edwin G.; Dorsa, Daniel.

In: Endocrinology, Vol. 138, No. 9, 1997, p. 4030-4033.

Research output: Contribution to journalArticle

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