Radioligand assay for Δ5-3β-hydroxysteroids I. 3β-hydroxy-5-androstene-17-one and its 3-sulfate

E. Nieschlag, Donald (Lynn) Loriaux, M. B. Lipsett

Research output: Contribution to journalArticle

59 Citations (Scopus)

Abstract

A specific, sensitive and reliable radioligand assay for plasma dehydroepiandrosterone (DHA) and its sulfate has been developed. Antisera were obtained by immunizing rabbits with a DHA-17-albumin conjugate. DHA was separated from cross-reacting Δ5-steroids by thin layer chromatography. DHA-sulfate was solvolyzed prior to chromatography. Separation of antibody bound and free steroid was achieved with γ-globulin-dextran-coated charcoal. The standard curve was linear on a logit-log plot from 0.1 to 10 ng.

Original languageEnglish (US)
Pages (from-to)669-679
Number of pages11
JournalSteroids
Volume19
Issue number5
DOIs
StatePublished - 1972
Externally publishedYes

Fingerprint

Androstenes
Hydroxysteroids
Radioligand Assay
Dehydroepiandrosterone Sulfate
Dehydroepiandrosterone
Sulfates
Assays
Steroids
Thin layer chromatography
Charcoal
Globulins
Thin Layer Chromatography
Chromatography
Dextrans
Immune Sera
Albumins
Rabbits
Plasmas
Antibodies

ASJC Scopus subject areas

  • Biochemistry
  • Clinical Biochemistry
  • Endocrinology
  • Molecular Biology
  • Organic Chemistry
  • Pharmacology

Cite this

Radioligand assay for Δ5-3β-hydroxysteroids I. 3β-hydroxy-5-androstene-17-one and its 3-sulfate. / Nieschlag, E.; Loriaux, Donald (Lynn); Lipsett, M. B.

In: Steroids, Vol. 19, No. 5, 1972, p. 669-679.

Research output: Contribution to journalArticle

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