Radioligand assay for Δ5-3β-hydroxysteroids I. 3β-hydroxy-5-androstene-17-one and its 3-sulfate

E. Nieschlag, Donald (Lynn) Loriaux, M. B. Lipsett

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Abstract

A specific, sensitive and reliable radioligand assay for plasma dehydroepiandrosterone (DHA) and its sulfate has been developed. Antisera were obtained by immunizing rabbits with a DHA-17-albumin conjugate. DHA was separated from cross-reacting Δ5-steroids by thin layer chromatography. DHA-sulfate was solvolyzed prior to chromatography. Separation of antibody bound and free steroid was achieved with γ-globulin-dextran-coated charcoal. The standard curve was linear on a logit-log plot from 0.1 to 10 ng.

Original languageEnglish (US)
Pages (from-to)669-679
Number of pages11
JournalSteroids
Volume19
Issue number5
DOIs
Publication statusPublished - 1972
Externally publishedYes

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ASJC Scopus subject areas

  • Biochemistry
  • Clinical Biochemistry
  • Endocrinology
  • Molecular Biology
  • Organic Chemistry
  • Pharmacology

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