Abstract
A specific, sensitive and reliable radioligand assay for plasma dehydroepiandrosterone (DHA) and its sulfate has been developed. Antisera were obtained by immunizing rabbits with a DHA-17-albumin conjugate. DHA was separated from cross-reacting Δ5-steroids by thin layer chromatography. DHA-sulfate was solvolyzed prior to chromatography. Separation of antibody bound and free steroid was achieved with γ-globulin-dextran-coated charcoal. The standard curve was linear on a logit-log plot from 0.1 to 10 ng.
Original language | English (US) |
---|---|
Pages (from-to) | 669-679 |
Number of pages | 11 |
Journal | Steroids |
Volume | 19 |
Issue number | 5 |
DOIs | |
State | Published - May 1972 |
Externally published | Yes |
ASJC Scopus subject areas
- Biochemistry
- Molecular Biology
- Endocrinology
- Pharmacology
- Clinical Biochemistry
- Organic Chemistry