Quisqualate activates N-methyl-D-aspartate receptor channels in hippocampal neurons maintained in culture

Timothy J. Grudt, Craig Jahr

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Whole-cell and single-channel patch-clamp recordings from hippocampal neurons in culture have been used to study the receptor channel selectivity of the glutamate analog quisqualate. The dose-response relationship of quisqualate acting at the N-methyl-D-aspartate (NMDA) receptor was measured as that portion of the whole-cell current activated by quisqualate that could be blocked by the addition of two NMDA antagonists, 5-fluoroindole-2-carboxylic acid, a competitive antagonist of the NMDA receptor-associated glycine site, and D-2-amino-5-phosphonovalerate, a competitive NMDA binding site antagonist. We found that quisqualate was 10-fold less potent than NMDA. In outsideout patches quisqualate activates single-channel events that range in conductance from 5 to 50 pS. The NMDA antagonists 5-fluoroindole-2-carboxylic acid and D-2-amino-5-phosphonovalerate completely blocked all of the 40-50-pS channel openings in the presence of quisqualate. These results indicate that quisqualate gates 40-50-pS events by activating NMDA receptor channels.

Original languageEnglish (US)
Pages (from-to)477-481
Number of pages5
JournalMolecular Pharmacology
Volume37
Issue number4
StatePublished - Apr 1990

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Quisqualic Acid
N-Methyl-D-Aspartate Receptors
Neurons
N-Methylaspartate
2-Amino-5-phosphonovalerate
Glycine
Glutamic Acid
Binding Sites

ASJC Scopus subject areas

  • Pharmacology

Cite this

Quisqualate activates N-methyl-D-aspartate receptor channels in hippocampal neurons maintained in culture. / Grudt, Timothy J.; Jahr, Craig.

In: Molecular Pharmacology, Vol. 37, No. 4, 04.1990, p. 477-481.

Research output: Contribution to journalArticle

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