Quantitative phase evaluation of dynamic changes on the cell membrane during laser microsurgery

Lingfeng Yu, Samarendra Mohanty, Gangjun Liu, Suzanne Genc, Myung K. Kim, Zhongping Chen, Michael W. Berns

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Citation (Scopus)

Abstract

Digital holographic microscopy allows determination of dynamic changes in the optical thickness profile of a transparent object with sub-wavelength accuracy. Here, we report a quantitative phase laser microsurgery system, which takes advantage of the precise optical manipulation by the laser microbeam and quantitative phase imaging by digital holographic microscopy with high spatial and temporal resolution. This system would enable absolute quantitation of localized alteration/damage to transparent phase objects, such as the cell membrane or intra-cellular structures, being exposed to the laser microbeam, which was not possible using conventional phase-contrast microscopy.

Original languageEnglish (US)
Title of host publicationProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume7182
DOIs
StatePublished - 2009
Externally publishedYes
EventImaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues VII - San Jose, CA, United States
Duration: Jan 26 2009Jan 28 2009

Other

OtherImaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues VII
CountryUnited States
CitySan Jose, CA
Period1/26/091/28/09

Fingerprint

Cell membranes
Microscopy
Microscopic examination
Membrane
microbeams
Laser
microscopy
Lasers
evaluation
Cell
Evaluation
lasers
Phase Contrast
phase contrast
temporal resolution
optical thickness
Manipulation
manipulators
Damage
spatial resolution

Keywords

  • Cellular damage
  • Laser scissors
  • Optical micromanipulation
  • Quantitative phase imaging

ASJC Scopus subject areas

  • Applied Mathematics
  • Computer Science Applications
  • Electrical and Electronic Engineering
  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics

Cite this

Yu, L., Mohanty, S., Liu, G., Genc, S., Kim, M. K., Chen, Z., & Berns, M. W. (2009). Quantitative phase evaluation of dynamic changes on the cell membrane during laser microsurgery. In Progress in Biomedical Optics and Imaging - Proceedings of SPIE (Vol. 7182). [718210] https://doi.org/10.1117/12.809761

Quantitative phase evaluation of dynamic changes on the cell membrane during laser microsurgery. / Yu, Lingfeng; Mohanty, Samarendra; Liu, Gangjun; Genc, Suzanne; Kim, Myung K.; Chen, Zhongping; Berns, Michael W.

Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 7182 2009. 718210.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Yu, L, Mohanty, S, Liu, G, Genc, S, Kim, MK, Chen, Z & Berns, MW 2009, Quantitative phase evaluation of dynamic changes on the cell membrane during laser microsurgery. in Progress in Biomedical Optics and Imaging - Proceedings of SPIE. vol. 7182, 718210, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues VII, San Jose, CA, United States, 1/26/09. https://doi.org/10.1117/12.809761
Yu L, Mohanty S, Liu G, Genc S, Kim MK, Chen Z et al. Quantitative phase evaluation of dynamic changes on the cell membrane during laser microsurgery. In Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 7182. 2009. 718210 https://doi.org/10.1117/12.809761
Yu, Lingfeng ; Mohanty, Samarendra ; Liu, Gangjun ; Genc, Suzanne ; Kim, Myung K. ; Chen, Zhongping ; Berns, Michael W. / Quantitative phase evaluation of dynamic changes on the cell membrane during laser microsurgery. Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 7182 2009.
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