Quantitative phase evaluation of dynamic changes on the cell membrane during laser microsurgery

Lingfeng Yu; Samarendra Mohanty; Gangjun Liu; Suzanne Genc; Myung K. Kim; Zhongping Chen; Michael W. Berns

doi:10.1117/12.809761

Quantitative phase evaluation of dynamic changes on the cell membrane during laser microsurgery

Lingfeng Yu, Samarendra Mohanty, Gangjun Liu, Suzanne Genc, Myung K. Kim, Zhongping Chen, Michael W. Berns

Research output: Contribution to journal › Conference article › peer-review

2 Scopus citations

Abstract

Digital holographic microscopy allows determination of dynamic changes in the optical thickness profile of a transparent object with sub-wavelength accuracy. Here, we report a quantitative phase laser microsurgery system, which takes advantage of the precise optical manipulation by the laser microbeam and quantitative phase imaging by digital holographic microscopy with high spatial and temporal resolution. This system would enable absolute quantitation of localized alteration/damage to transparent phase objects, such as the cell membrane or intra-cellular structures, being exposed to the laser microbeam, which was not possible using conventional phase-contrast microscopy.

Original language	English (US)
Article number	718210
Journal	Progress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume	7182
DOIs	https://doi.org/10.1117/12.809761
State	Published - 2009
Externally published	Yes
Event	Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues VII - San Jose, CA, United States Duration: Jan 26 2009 → Jan 28 2009

Keywords

Cellular damage
Laser scissors
Optical micromanipulation
Quantitative phase imaging

ASJC Scopus subject areas

Electronic, Optical and Magnetic Materials
Atomic and Molecular Physics, and Optics
Biomaterials
Radiology Nuclear Medicine and imaging

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title = "Quantitative phase evaluation of dynamic changes on the cell membrane during laser microsurgery",

abstract = "Digital holographic microscopy allows determination of dynamic changes in the optical thickness profile of a transparent object with sub-wavelength accuracy. Here, we report a quantitative phase laser microsurgery system, which takes advantage of the precise optical manipulation by the laser microbeam and quantitative phase imaging by digital holographic microscopy with high spatial and temporal resolution. This system would enable absolute quantitation of localized alteration/damage to transparent phase objects, such as the cell membrane or intra-cellular structures, being exposed to the laser microbeam, which was not possible using conventional phase-contrast microscopy.",

keywords = "Cellular damage, Laser scissors, Optical micromanipulation, Quantitative phase imaging",

author = "Lingfeng Yu and Samarendra Mohanty and Gangjun Liu and Suzanne Genc and Kim, {Myung K.} and Zhongping Chen and Berns, {Michael W.}",

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doi = "10.1117/12.809761",

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T1 - Quantitative phase evaluation of dynamic changes on the cell membrane during laser microsurgery

AU - Yu, Lingfeng

AU - Mohanty, Samarendra

AU - Liu, Gangjun

AU - Genc, Suzanne

AU - Kim, Myung K.

AU - Chen, Zhongping

AU - Berns, Michael W.

PY - 2009

Y1 - 2009

N2 - Digital holographic microscopy allows determination of dynamic changes in the optical thickness profile of a transparent object with sub-wavelength accuracy. Here, we report a quantitative phase laser microsurgery system, which takes advantage of the precise optical manipulation by the laser microbeam and quantitative phase imaging by digital holographic microscopy with high spatial and temporal resolution. This system would enable absolute quantitation of localized alteration/damage to transparent phase objects, such as the cell membrane or intra-cellular structures, being exposed to the laser microbeam, which was not possible using conventional phase-contrast microscopy.

AB - Digital holographic microscopy allows determination of dynamic changes in the optical thickness profile of a transparent object with sub-wavelength accuracy. Here, we report a quantitative phase laser microsurgery system, which takes advantage of the precise optical manipulation by the laser microbeam and quantitative phase imaging by digital holographic microscopy with high spatial and temporal resolution. This system would enable absolute quantitation of localized alteration/damage to transparent phase objects, such as the cell membrane or intra-cellular structures, being exposed to the laser microbeam, which was not possible using conventional phase-contrast microscopy.

KW - Cellular damage

KW - Laser scissors

KW - Optical micromanipulation

KW - Quantitative phase imaging

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JF - Progress in Biomedical Optics and Imaging - Proceedings of SPIE

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ER -

Quantitative phase evaluation of dynamic changes on the cell membrane during laser microsurgery

Abstract

Keywords

ASJC Scopus subject areas

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