Quantitative phase evaluation of dynamic changes on cell membrane during laser microsurgery

Lingfeng Yu, Samarendra Mohanty, Gangjun Liu, Suzanne Genc, Zhongping Chen, Michael W. Berns

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

The ability to inject exogenous material as well as to alter subcellular structures in a minimally invasive manner using a laser microbeam has been useful for cell biologists to study the structure-function relationship in complex biological systems. We describe a quantitative phase laser microsurgery system, which takes advantage of the combination of laser microirradiation and short-coherence interference microscopy. Using this method, quantitative phase images and the dynamic changes of phase during the process of laser microsurgery of red blood cells (RBCs) can be evaluated in real time. This system would enable absolute quantitation of localized alteration/damage to transparent phase objects, such as the cell membrane or intracellular structures, being exposed to the laser microbeam. Such quantitation was not possible using conventional phase-contrast microscopy.

Original languageEnglish (US)
Article number050508
JournalJournal of Biomedical Optics
Volume13
Issue number5
DOIs
StatePublished - 2008
Externally publishedYes

Fingerprint

Cell membranes
Lasers
evaluation
microbeams
lasers
Microscopic examination
microscopy
membrane structures
phase contrast
erythrocytes
Biological systems
Blood
Cells
damage
interference
cells

Keywords

  • cellular damage
  • laser scissors
  • optical micromanipulation
  • quantitative phase imaging
  • short-coherence interference microscopy

ASJC Scopus subject areas

  • Biomedical Engineering
  • Biomaterials
  • Electronic, Optical and Magnetic Materials
  • Atomic and Molecular Physics, and Optics

Cite this

Quantitative phase evaluation of dynamic changes on cell membrane during laser microsurgery. / Yu, Lingfeng; Mohanty, Samarendra; Liu, Gangjun; Genc, Suzanne; Chen, Zhongping; Berns, Michael W.

In: Journal of Biomedical Optics, Vol. 13, No. 5, 050508, 2008.

Research output: Contribution to journalArticle

Yu, Lingfeng ; Mohanty, Samarendra ; Liu, Gangjun ; Genc, Suzanne ; Chen, Zhongping ; Berns, Michael W. / Quantitative phase evaluation of dynamic changes on cell membrane during laser microsurgery. In: Journal of Biomedical Optics. 2008 ; Vol. 13, No. 5.
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