Quantitative measurement of human interleukin 2 receptor levels with intact and detergent-solubilized human T-cells

Steven K. Dower, Steven Hefeneider, Alan R. Alpert, David L. Urdal

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

2A3 monoclonal antibody (γ1, κ) is a novel high-affinity reagent for detecting the human interleukin 2 (IL-2) receptor. The antibody inhibits IL-2 binding to its receptor and is an antagonist of IL-2 action. Detailed analysis of the mechanism of binding of the IgG, (Fab')2 and Fab' of 2A3 antibody shows that the bivalent species cross-link on the cell surface when bound. Measurements of IL-2 receptor expression on digitonin-permeabilized cells suggest that the intracellular pool of receptors is small. The antibody will bind to IL-2 receptors on glutaraldehyde-fixed cells in the presence of Triton X-100. This property is used in designing an assay for quantitative measurements of IL-2 receptor concn in solution. This assay can be used to monitor receptor protein during purification to homogeneity.

Original languageEnglish (US)
Pages (from-to)937-947
Number of pages11
JournalMolecular Immunology
Volume22
Issue number8
DOIs
StatePublished - 1985
Externally publishedYes

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Interleukin-2 Receptors
Detergents
T-Lymphocytes
Interleukin-2
Antibodies
Digitonin
Octoxynol
Glutaral
Immunoglobulin G
Monoclonal Antibodies
Proteins

ASJC Scopus subject areas

  • Molecular Biology
  • Immunology

Cite this

Quantitative measurement of human interleukin 2 receptor levels with intact and detergent-solubilized human T-cells. / Dower, Steven K.; Hefeneider, Steven; Alpert, Alan R.; Urdal, David L.

In: Molecular Immunology, Vol. 22, No. 8, 1985, p. 937-947.

Research output: Contribution to journalArticle

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