Abstract
2A3 monoclonal antibody (γ1, κ) is a novel high-affinity reagent for detecting the human interleukin 2 (IL-2) receptor. The antibody inhibits IL-2 binding to its receptor and is an antagonist of IL-2 action. Detailed analysis of the mechanism of binding of the IgG, (Fab')2 and Fab' of 2A3 antibody shows that the bivalent species cross-link on the cell surface when bound. Measurements of IL-2 receptor expression on digitonin-permeabilized cells suggest that the intracellular pool of receptors is small. The antibody will bind to IL-2 receptors on glutaraldehyde-fixed cells in the presence of Triton X-100. This property is used in designing an assay for quantitative measurements of IL-2 receptor concn in solution. This assay can be used to monitor receptor protein during purification to homogeneity.
Original language | English (US) |
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Pages (from-to) | 937-947 |
Number of pages | 11 |
Journal | Molecular Immunology |
Volume | 22 |
Issue number | 8 |
DOIs | |
State | Published - Aug 1985 |
Externally published | Yes |
ASJC Scopus subject areas
- Immunology
- Molecular Biology