Quantitative measurement of human interleukin 2 receptor levels with intact and detergent-solubilized human T-cells

Steven K. Dower, Steven H. Hefeneider, Alan R. Alpert, David L. Urdal

Research output: Contribution to journalArticle

18 Scopus citations

Abstract

2A3 monoclonal antibody (γ1, κ) is a novel high-affinity reagent for detecting the human interleukin 2 (IL-2) receptor. The antibody inhibits IL-2 binding to its receptor and is an antagonist of IL-2 action. Detailed analysis of the mechanism of binding of the IgG, (Fab')2 and Fab' of 2A3 antibody shows that the bivalent species cross-link on the cell surface when bound. Measurements of IL-2 receptor expression on digitonin-permeabilized cells suggest that the intracellular pool of receptors is small. The antibody will bind to IL-2 receptors on glutaraldehyde-fixed cells in the presence of Triton X-100. This property is used in designing an assay for quantitative measurements of IL-2 receptor concn in solution. This assay can be used to monitor receptor protein during purification to homogeneity.

Original languageEnglish (US)
Pages (from-to)937-947
Number of pages11
JournalMolecular Immunology
Volume22
Issue number8
DOIs
StatePublished - Aug 1985

ASJC Scopus subject areas

  • Immunology
  • Molecular Biology

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