Quantitative fluorescence microscopy yields distributions of concentration and photobleaching rate in 5ALA-induced protoporphyrin IX in cell population

Kunio Awazu, Steven Jacques

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Citation (Scopus)

Abstract

The variation in photodynamic dose achieved with protoporphyrin Ix (PPIX) and laser radiation was assessed in a cancer cell culture. the distributions of initial PPIX concentration, photobleaching light dose, and photodynamic dose were measured on 124 individual cells (rat mammary cancer cell line, MTF7) using video fluorescence microscopy.

Original languageEnglish (US)
Title of host publicationProceedings of SPIE - The International Society for Optical Engineering
EditorsThomas J. Dougherty
Pages77-84
Number of pages8
Volume2392
StatePublished - 1995
Externally publishedYes
EventOptical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy IV - San Jose, CA, USA
Duration: Feb 4 1995Feb 5 1995

Other

OtherOptical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy IV
CitySan Jose, CA, USA
Period2/4/952/5/95

Fingerprint

Photobleaching
Fluorescence microscopy
Dosimetry
Cells
microscopy
dosage
fluorescence
Laser radiation
cancer
Cell culture
Rats
cultured cells
rats
laser beams
cells

ASJC Scopus subject areas

  • Electrical and Electronic Engineering
  • Condensed Matter Physics

Cite this

Awazu, K., & Jacques, S. (1995). Quantitative fluorescence microscopy yields distributions of concentration and photobleaching rate in 5ALA-induced protoporphyrin IX in cell population. In T. J. Dougherty (Ed.), Proceedings of SPIE - The International Society for Optical Engineering (Vol. 2392, pp. 77-84)

Quantitative fluorescence microscopy yields distributions of concentration and photobleaching rate in 5ALA-induced protoporphyrin IX in cell population. / Awazu, Kunio; Jacques, Steven.

Proceedings of SPIE - The International Society for Optical Engineering. ed. / Thomas J. Dougherty. Vol. 2392 1995. p. 77-84.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Awazu, K & Jacques, S 1995, Quantitative fluorescence microscopy yields distributions of concentration and photobleaching rate in 5ALA-induced protoporphyrin IX in cell population. in TJ Dougherty (ed.), Proceedings of SPIE - The International Society for Optical Engineering. vol. 2392, pp. 77-84, Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy IV, San Jose, CA, USA, 2/4/95.
Awazu K, Jacques S. Quantitative fluorescence microscopy yields distributions of concentration and photobleaching rate in 5ALA-induced protoporphyrin IX in cell population. In Dougherty TJ, editor, Proceedings of SPIE - The International Society for Optical Engineering. Vol. 2392. 1995. p. 77-84
Awazu, Kunio ; Jacques, Steven. / Quantitative fluorescence microscopy yields distributions of concentration and photobleaching rate in 5ALA-induced protoporphyrin IX in cell population. Proceedings of SPIE - The International Society for Optical Engineering. editor / Thomas J. Dougherty. Vol. 2392 1995. pp. 77-84
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