Purified hematopoietic stem cells can differentiate into hepatocytes in vivo

Eric Lagasse, Heather Connors, Muhsen Al-Dhalimy, Michael Reitsma, Monika Dohse, Linda Osborne, Xin Wang, Milton Finegold, Irving L. Weissman, Markus Grompe

Research output: Contribution to journalArticle

2063 Citations (Scopus)

Abstract

The characterization of hepatic progenitor cells is of great scientific and clinical interest. Here we report that intravenous injection of adult bone marrow cells in the FAH(-/-) mouse, an animal model of tyrosinemia type I, rescued the mouse and restored the biochemical function of its liver. Moreover, within bone marrow, only rigorously purified hematopoietic stem cells gave rise to donor-derived hematopoietic and hepatic regeneration. This result seems to contradict the conventional assumptions of the germ layer origins of tissues such as the liver, and raises the question of whether the cells of the hematopoietic stem cell phenotype are pluripotent hematopoietic cells that retain the ability to transdifferentiate, or whether they are more primitive multipotent cells.

Original languageEnglish (US)
Pages (from-to)1229-1234
Number of pages6
JournalNature Medicine
Volume6
Issue number11
DOIs
StatePublished - 2000

Fingerprint

Hematopoietic Stem Cells
Stem cells
Liver
Hepatocytes
Bone
Tyrosinemias
Germ Layers
Animals
Cells
Tissue
Intravenous Injections
Bone Marrow Cells
Regeneration
Stem Cells
Animal Models
Bone Marrow
Phenotype

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Lagasse, E., Connors, H., Al-Dhalimy, M., Reitsma, M., Dohse, M., Osborne, L., ... Grompe, M. (2000). Purified hematopoietic stem cells can differentiate into hepatocytes in vivo. Nature Medicine, 6(11), 1229-1234. https://doi.org/10.1038/81326

Purified hematopoietic stem cells can differentiate into hepatocytes in vivo. / Lagasse, Eric; Connors, Heather; Al-Dhalimy, Muhsen; Reitsma, Michael; Dohse, Monika; Osborne, Linda; Wang, Xin; Finegold, Milton; Weissman, Irving L.; Grompe, Markus.

In: Nature Medicine, Vol. 6, No. 11, 2000, p. 1229-1234.

Research output: Contribution to journalArticle

Lagasse, E, Connors, H, Al-Dhalimy, M, Reitsma, M, Dohse, M, Osborne, L, Wang, X, Finegold, M, Weissman, IL & Grompe, M 2000, 'Purified hematopoietic stem cells can differentiate into hepatocytes in vivo', Nature Medicine, vol. 6, no. 11, pp. 1229-1234. https://doi.org/10.1038/81326
Lagasse E, Connors H, Al-Dhalimy M, Reitsma M, Dohse M, Osborne L et al. Purified hematopoietic stem cells can differentiate into hepatocytes in vivo. Nature Medicine. 2000;6(11):1229-1234. https://doi.org/10.1038/81326
Lagasse, Eric ; Connors, Heather ; Al-Dhalimy, Muhsen ; Reitsma, Michael ; Dohse, Monika ; Osborne, Linda ; Wang, Xin ; Finegold, Milton ; Weissman, Irving L. ; Grompe, Markus. / Purified hematopoietic stem cells can differentiate into hepatocytes in vivo. In: Nature Medicine. 2000 ; Vol. 6, No. 11. pp. 1229-1234.
@article{ade244336d9348ae87b5b83ae334d1de,
title = "Purified hematopoietic stem cells can differentiate into hepatocytes in vivo",
abstract = "The characterization of hepatic progenitor cells is of great scientific and clinical interest. Here we report that intravenous injection of adult bone marrow cells in the FAH(-/-) mouse, an animal model of tyrosinemia type I, rescued the mouse and restored the biochemical function of its liver. Moreover, within bone marrow, only rigorously purified hematopoietic stem cells gave rise to donor-derived hematopoietic and hepatic regeneration. This result seems to contradict the conventional assumptions of the germ layer origins of tissues such as the liver, and raises the question of whether the cells of the hematopoietic stem cell phenotype are pluripotent hematopoietic cells that retain the ability to transdifferentiate, or whether they are more primitive multipotent cells.",
author = "Eric Lagasse and Heather Connors and Muhsen Al-Dhalimy and Michael Reitsma and Monika Dohse and Linda Osborne and Xin Wang and Milton Finegold and Weissman, {Irving L.} and Markus Grompe",
year = "2000",
doi = "10.1038/81326",
language = "English (US)",
volume = "6",
pages = "1229--1234",
journal = "Nature Medicine",
issn = "1078-8956",
publisher = "Nature Publishing Group",
number = "11",

}

TY - JOUR

T1 - Purified hematopoietic stem cells can differentiate into hepatocytes in vivo

AU - Lagasse, Eric

AU - Connors, Heather

AU - Al-Dhalimy, Muhsen

AU - Reitsma, Michael

AU - Dohse, Monika

AU - Osborne, Linda

AU - Wang, Xin

AU - Finegold, Milton

AU - Weissman, Irving L.

AU - Grompe, Markus

PY - 2000

Y1 - 2000

N2 - The characterization of hepatic progenitor cells is of great scientific and clinical interest. Here we report that intravenous injection of adult bone marrow cells in the FAH(-/-) mouse, an animal model of tyrosinemia type I, rescued the mouse and restored the biochemical function of its liver. Moreover, within bone marrow, only rigorously purified hematopoietic stem cells gave rise to donor-derived hematopoietic and hepatic regeneration. This result seems to contradict the conventional assumptions of the germ layer origins of tissues such as the liver, and raises the question of whether the cells of the hematopoietic stem cell phenotype are pluripotent hematopoietic cells that retain the ability to transdifferentiate, or whether they are more primitive multipotent cells.

AB - The characterization of hepatic progenitor cells is of great scientific and clinical interest. Here we report that intravenous injection of adult bone marrow cells in the FAH(-/-) mouse, an animal model of tyrosinemia type I, rescued the mouse and restored the biochemical function of its liver. Moreover, within bone marrow, only rigorously purified hematopoietic stem cells gave rise to donor-derived hematopoietic and hepatic regeneration. This result seems to contradict the conventional assumptions of the germ layer origins of tissues such as the liver, and raises the question of whether the cells of the hematopoietic stem cell phenotype are pluripotent hematopoietic cells that retain the ability to transdifferentiate, or whether they are more primitive multipotent cells.

UR - http://www.scopus.com/inward/record.url?scp=0033694301&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0033694301&partnerID=8YFLogxK

U2 - 10.1038/81326

DO - 10.1038/81326

M3 - Article

C2 - 11062533

AN - SCOPUS:0033694301

VL - 6

SP - 1229

EP - 1234

JO - Nature Medicine

JF - Nature Medicine

SN - 1078-8956

IS - 11

ER -