Purification and partial characterization of an ice nucleator protein from the intertidal gastropod, Melampus bidentatus

Dana Madison, Mona M. Scrofano, Robert C. Ireland, Stephen H. Loomis

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

An ice nucleator protein has been purified from the intertidal gastropod Melampus bidentatus by a combination of gel filtration and ion exchange chromatography. Partial characterization of this ice nucleator protein indicates that in the native form, it exists as an aggregate with a molecular weight in excess of 600 kDa. SDS-PAGE analysis indicates that this aggregate is composed of two low molecular weight protein species of approximately 16.6 and 17.4 kDa. Biochemical analysis indicates that it is neither a lipoprotein nor a glycoprotein. The amino acid composition indicates that it contains a high percentage of polar amino acids with Asx and Glx representing over 20 mol% of the protein. The characteristics of this molluskan ice nucleator protein are compared with those of purified ice nucleator proteins obtained from bacteria and various species of insects.

Original languageEnglish (US)
Pages (from-to)483-490
Number of pages8
JournalCryobiology
Volume28
Issue number5
DOIs
StatePublished - 1991
Externally publishedYes

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Gastropoda
Ice
Purification
ice
Proteins
proteins
protein aggregates
Molecular Weight
Molecular weight
molecular weight
Amino Acids
Ion Exchange Chromatography
ion exchange chromatography
Chromatography
amino acid composition
lipoproteins
Lipoproteins
Gel Chromatography
Insects
glycoproteins

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)

Cite this

Purification and partial characterization of an ice nucleator protein from the intertidal gastropod, Melampus bidentatus. / Madison, Dana; Scrofano, Mona M.; Ireland, Robert C.; Loomis, Stephen H.

In: Cryobiology, Vol. 28, No. 5, 1991, p. 483-490.

Research output: Contribution to journalArticle

Madison, Dana ; Scrofano, Mona M. ; Ireland, Robert C. ; Loomis, Stephen H. / Purification and partial characterization of an ice nucleator protein from the intertidal gastropod, Melampus bidentatus. In: Cryobiology. 1991 ; Vol. 28, No. 5. pp. 483-490.
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