@article{515c0da7355f4ea3b0d515ef0655c872,
title = "Proteome Profiling of 1 to 5 Spiked Circulating Tumor Cells Isolated from Whole Blood Using Immunodensity Enrichment, Laser Capture Microdissection, Nanodroplet Sample Processing, and Ultrasensitive nanoLC-MS",
abstract = "Proteome profiling of circulating tumor cells (CTCs) can provide crucial insight into disease progression and the role of CTCs in tumor metastasis. We describe an integrated workflow to measure global protein expression in 1-5 spiked CTCs enriched from whole blood by immunodensity gradient centrifugation. Enriched CTCs were purified and collected by laser capture microdissection, prepared using a recently developed nanodroplet-based processing platform (nanoPOTS), and finally analyzed by ultrasensitive nanoLC-MS/MS. The workflow was capable of identifying an average of 164 and 607 protein groups from samples comprising 1 and 5 LNCaP cells, respectively, that were isolated from human whole blood. A panel of prostate cancer-specific proteins were identified and quantified, which was used to differentiate between spiked CTCs and white blood cells.",
author = "Ying Zhu and Jennifer Podolak and Rui Zhao and Shukla, {Anil K.} and Moore, {Ronald J.} and Thomas, {George V.} and Kelly, {Ryan T.}",
note = "Funding Information: This work was supported by NIH Grants R33 CA225248 (R.T.K.) and R21 EB020976 (R.T.K.), the Pacific Northwest Prostate Cancer Grant SPORE/NCI P50 CA097186 (G.V.T), the OHSU-Knight Cancer Institute (G.V.T), Earth & Biological Sciences Directorate Mission Seed under the Laboratory Directed Research and Development Program at PNNL (Y.Z.), and the Precision Medicine Innovation Co-Laboratory (PMedIC), a joint research collaboration of OHSU and PNNL. A portion of this research was performed using EMSL, a national scientific user facility sponsored by the Department of Energy{\textquoteright}s Office of Biological and Environmental Research and located at PNNL. Publisher Copyright: {\textcopyright} Copyright 2018 American Chemical Society.",
year = "2018",
month = oct,
day = "16",
doi = "10.1021/acs.analchem.8b03268",
language = "English (US)",
volume = "90",
pages = "11756--11759",
journal = "Analytical Chemistry",
issn = "0003-2700",
number = "20",
}