Proteome Profiling of 1 to 5 Spiked Circulating Tumor Cells Isolated from Whole Blood Using Immunodensity Enrichment, Laser Capture Microdissection, Nanodroplet Sample Processing, and Ultrasensitive nanoLC-MS

Ying Zhu, Jennifer Podolak, Rui Zhao, Anil K. Shukla, Ronald J. Moore, George Thomas, Ryan T. Kelly

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Proteome profiling of circulating tumor cells (CTCs) can provide crucial insight into disease progression and the role of CTCs in tumor metastasis. We describe an integrated workflow to measure global protein expression in 1-5 spiked CTCs enriched from whole blood by immunodensity gradient centrifugation. Enriched CTCs were purified and collected by laser capture microdissection, prepared using a recently developed nanodroplet-based processing platform (nanoPOTS), and finally analyzed by ultrasensitive nanoLC-MS/MS. The workflow was capable of identifying an average of 164 and 607 protein groups from samples comprising 1 and 5 LNCaP cells, respectively, that were isolated from human whole blood. A panel of prostate cancer-specific proteins were identified and quantified, which was used to differentiate between spiked CTCs and white blood cells.

Original languageEnglish (US)
Pages (from-to)11756-11759
Number of pages4
JournalAnalytical Chemistry
Volume90
Issue number20
DOIs
StatePublished - Oct 16 2018

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Microdissection
Proteome
Tumors
Blood
Cells
Lasers
Processing
Proteins
Centrifugation

ASJC Scopus subject areas

  • Analytical Chemistry

Cite this

Proteome Profiling of 1 to 5 Spiked Circulating Tumor Cells Isolated from Whole Blood Using Immunodensity Enrichment, Laser Capture Microdissection, Nanodroplet Sample Processing, and Ultrasensitive nanoLC-MS. / Zhu, Ying; Podolak, Jennifer; Zhao, Rui; Shukla, Anil K.; Moore, Ronald J.; Thomas, George; Kelly, Ryan T.

In: Analytical Chemistry, Vol. 90, No. 20, 16.10.2018, p. 11756-11759.

Research output: Contribution to journalArticle

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AU - Thomas, George

AU - Kelly, Ryan T.

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