Proteome of Salmonella enterica serotype Typhimurium grown in a low Mg2+/pH medium

Liang Shi, Charles Ansong, Heather Smallwood, Leah Rommereim, Jason E. McDermott, Heather M. Brewer, Angela D. Norbeck, Ronald C. Taylor, Jean Gustin, Fred Heffron, Richard D. Smith, Joshua N. Adkins

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

To determine the impact of a low Mg2+/pH defined growth medium (MgM) on the proteome of Salmonella enterica serotype Typhimurium, we cultured S. Typhimurium cells in the medium under two different conditions termed MgM Shock and MgM Dilution and then comparatively analyzed the bacterial cells harvested from these conditions by a global proteomic approach. Proteomic results showed that MgM Shock and MgM Dilution differentially affected the S. Typhimurium proteome. MgM Shock induced a group of proteins whose induction usually occurred at low O2 level, while MgM Dilution induced those related to the type III secretion system (T3SS) of Salmonella Pathogenicity Island 2 (SPI2) and those involved in thiamine or biotin biosynthesis. The metabolic state of the S. Typhimurium cells grown under MgM Shock condition also differed significantly from that under MgM Dilution condition. Western blot analysis not only confirmed the proteomic results, but also showed that the abundances of SPI2-T3SS proteins SsaQ and SseE and biotin biosynthesis proteins BioB and BioD increased after S. Typhimurium infection of RAW 264.7 macrophages. Deletion of the gene encoding BioB reduced the bacterial ability to replicate inside the macrophages, suggesting a biotin-limited environment encountered by S. Typhimurium within RAW 264.7 macrophages.

Original languageEnglish (US)
Pages (from-to)388-397
Number of pages10
JournalJournal of Proteomics and Bioinformatics
Volume2
Issue number9
DOIs
StatePublished - Sep 2009

Fingerprint

Salmonella
Salmonella enterica
Proteome
Dilution
Macrophages
Shock
Biotin
Proteomics
Proteins
Genomic Islands
Biosynthesis
Gene encoding
Thiamine
Gene Deletion
Protein Biosynthesis
Western Blotting
Cells
Serogroup
Growth
Infection

Keywords

  • Biotin biosynthesis
  • Low Mg+/pH defined growth medium
  • Salmonella enterica serotype typhimurium
  • Salmonella pathogenicity Island 2
  • Salmonella-containing vacuole
  • Type III secretion system

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Molecular Biology
  • Computer Science Applications

Cite this

Shi, L., Ansong, C., Smallwood, H., Rommereim, L., McDermott, J. E., Brewer, H. M., ... Adkins, J. N. (2009). Proteome of Salmonella enterica serotype Typhimurium grown in a low Mg2+/pH medium. Journal of Proteomics and Bioinformatics, 2(9), 388-397. https://doi.org/10.4172/jpb.1000099

Proteome of Salmonella enterica serotype Typhimurium grown in a low Mg2+/pH medium. / Shi, Liang; Ansong, Charles; Smallwood, Heather; Rommereim, Leah; McDermott, Jason E.; Brewer, Heather M.; Norbeck, Angela D.; Taylor, Ronald C.; Gustin, Jean; Heffron, Fred; Smith, Richard D.; Adkins, Joshua N.

In: Journal of Proteomics and Bioinformatics, Vol. 2, No. 9, 09.2009, p. 388-397.

Research output: Contribution to journalArticle

Shi, L, Ansong, C, Smallwood, H, Rommereim, L, McDermott, JE, Brewer, HM, Norbeck, AD, Taylor, RC, Gustin, J, Heffron, F, Smith, RD & Adkins, JN 2009, 'Proteome of Salmonella enterica serotype Typhimurium grown in a low Mg2+/pH medium', Journal of Proteomics and Bioinformatics, vol. 2, no. 9, pp. 388-397. https://doi.org/10.4172/jpb.1000099
Shi, Liang ; Ansong, Charles ; Smallwood, Heather ; Rommereim, Leah ; McDermott, Jason E. ; Brewer, Heather M. ; Norbeck, Angela D. ; Taylor, Ronald C. ; Gustin, Jean ; Heffron, Fred ; Smith, Richard D. ; Adkins, Joshua N. / Proteome of Salmonella enterica serotype Typhimurium grown in a low Mg2+/pH medium. In: Journal of Proteomics and Bioinformatics. 2009 ; Vol. 2, No. 9. pp. 388-397.
@article{a659f856aa49455e9bc9bb3941038fdc,
title = "Proteome of Salmonella enterica serotype Typhimurium grown in a low Mg2+/pH medium",
abstract = "To determine the impact of a low Mg2+/pH defined growth medium (MgM) on the proteome of Salmonella enterica serotype Typhimurium, we cultured S. Typhimurium cells in the medium under two different conditions termed MgM Shock and MgM Dilution and then comparatively analyzed the bacterial cells harvested from these conditions by a global proteomic approach. Proteomic results showed that MgM Shock and MgM Dilution differentially affected the S. Typhimurium proteome. MgM Shock induced a group of proteins whose induction usually occurred at low O2 level, while MgM Dilution induced those related to the type III secretion system (T3SS) of Salmonella Pathogenicity Island 2 (SPI2) and those involved in thiamine or biotin biosynthesis. The metabolic state of the S. Typhimurium cells grown under MgM Shock condition also differed significantly from that under MgM Dilution condition. Western blot analysis not only confirmed the proteomic results, but also showed that the abundances of SPI2-T3SS proteins SsaQ and SseE and biotin biosynthesis proteins BioB and BioD increased after S. Typhimurium infection of RAW 264.7 macrophages. Deletion of the gene encoding BioB reduced the bacterial ability to replicate inside the macrophages, suggesting a biotin-limited environment encountered by S. Typhimurium within RAW 264.7 macrophages.",
keywords = "Biotin biosynthesis, Low Mg+/pH defined growth medium, Salmonella enterica serotype typhimurium, Salmonella pathogenicity Island 2, Salmonella-containing vacuole, Type III secretion system",
author = "Liang Shi and Charles Ansong and Heather Smallwood and Leah Rommereim and McDermott, {Jason E.} and Brewer, {Heather M.} and Norbeck, {Angela D.} and Taylor, {Ronald C.} and Jean Gustin and Fred Heffron and Smith, {Richard D.} and Adkins, {Joshua N.}",
year = "2009",
month = "9",
doi = "10.4172/jpb.1000099",
language = "English (US)",
volume = "2",
pages = "388--397",
journal = "Journal of Proteomics and Bioinformatics",
issn = "0974-276X",
publisher = "Omics Publishing Group",
number = "9",

}

TY - JOUR

T1 - Proteome of Salmonella enterica serotype Typhimurium grown in a low Mg2+/pH medium

AU - Shi, Liang

AU - Ansong, Charles

AU - Smallwood, Heather

AU - Rommereim, Leah

AU - McDermott, Jason E.

AU - Brewer, Heather M.

AU - Norbeck, Angela D.

AU - Taylor, Ronald C.

AU - Gustin, Jean

AU - Heffron, Fred

AU - Smith, Richard D.

AU - Adkins, Joshua N.

PY - 2009/9

Y1 - 2009/9

N2 - To determine the impact of a low Mg2+/pH defined growth medium (MgM) on the proteome of Salmonella enterica serotype Typhimurium, we cultured S. Typhimurium cells in the medium under two different conditions termed MgM Shock and MgM Dilution and then comparatively analyzed the bacterial cells harvested from these conditions by a global proteomic approach. Proteomic results showed that MgM Shock and MgM Dilution differentially affected the S. Typhimurium proteome. MgM Shock induced a group of proteins whose induction usually occurred at low O2 level, while MgM Dilution induced those related to the type III secretion system (T3SS) of Salmonella Pathogenicity Island 2 (SPI2) and those involved in thiamine or biotin biosynthesis. The metabolic state of the S. Typhimurium cells grown under MgM Shock condition also differed significantly from that under MgM Dilution condition. Western blot analysis not only confirmed the proteomic results, but also showed that the abundances of SPI2-T3SS proteins SsaQ and SseE and biotin biosynthesis proteins BioB and BioD increased after S. Typhimurium infection of RAW 264.7 macrophages. Deletion of the gene encoding BioB reduced the bacterial ability to replicate inside the macrophages, suggesting a biotin-limited environment encountered by S. Typhimurium within RAW 264.7 macrophages.

AB - To determine the impact of a low Mg2+/pH defined growth medium (MgM) on the proteome of Salmonella enterica serotype Typhimurium, we cultured S. Typhimurium cells in the medium under two different conditions termed MgM Shock and MgM Dilution and then comparatively analyzed the bacterial cells harvested from these conditions by a global proteomic approach. Proteomic results showed that MgM Shock and MgM Dilution differentially affected the S. Typhimurium proteome. MgM Shock induced a group of proteins whose induction usually occurred at low O2 level, while MgM Dilution induced those related to the type III secretion system (T3SS) of Salmonella Pathogenicity Island 2 (SPI2) and those involved in thiamine or biotin biosynthesis. The metabolic state of the S. Typhimurium cells grown under MgM Shock condition also differed significantly from that under MgM Dilution condition. Western blot analysis not only confirmed the proteomic results, but also showed that the abundances of SPI2-T3SS proteins SsaQ and SseE and biotin biosynthesis proteins BioB and BioD increased after S. Typhimurium infection of RAW 264.7 macrophages. Deletion of the gene encoding BioB reduced the bacterial ability to replicate inside the macrophages, suggesting a biotin-limited environment encountered by S. Typhimurium within RAW 264.7 macrophages.

KW - Biotin biosynthesis

KW - Low Mg+/pH defined growth medium

KW - Salmonella enterica serotype typhimurium

KW - Salmonella pathogenicity Island 2

KW - Salmonella-containing vacuole

KW - Type III secretion system

UR - http://www.scopus.com/inward/record.url?scp=75649151555&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=75649151555&partnerID=8YFLogxK

U2 - 10.4172/jpb.1000099

DO - 10.4172/jpb.1000099

M3 - Article

VL - 2

SP - 388

EP - 397

JO - Journal of Proteomics and Bioinformatics

JF - Journal of Proteomics and Bioinformatics

SN - 0974-276X

IS - 9

ER -