Proteome of Salmonella enterica serotype Typhimurium grown in a low Mg2+/pH medium

Liang Shi, Charles Ansong, Heather Smallwood, Leah Rommereim, Jason E. McDermott, Heather M. Brewer, Angela D. Norbeck, Ronald C. Taylor, Jean K. Gustin, Fred Heffron, Richard D. Smith, Joshua N. Adkins

Research output: Contribution to journalArticle

16 Scopus citations

Abstract

To determine the impact of a low Mg2+/pH defined growth medium (MgM) on the proteome of Salmonella enterica serotype Typhimurium, we cultured S. Typhimurium cells in the medium under two different conditions termed MgM Shock and MgM Dilution and then comparatively analyzed the bacterial cells harvested from these conditions by a global proteomic approach. Proteomic results showed that MgM Shock and MgM Dilution differentially affected the S. Typhimurium proteome. MgM Shock induced a group of proteins whose induction usually occurred at low O2 level, while MgM Dilution induced those related to the type III secretion system (T3SS) of Salmonella Pathogenicity Island 2 (SPI2) and those involved in thiamine or biotin biosynthesis. The metabolic state of the S. Typhimurium cells grown under MgM Shock condition also differed significantly from that under MgM Dilution condition. Western blot analysis not only confirmed the proteomic results, but also showed that the abundances of SPI2-T3SS proteins SsaQ and SseE and biotin biosynthesis proteins BioB and BioD increased after S. Typhimurium infection of RAW 264.7 macrophages. Deletion of the gene encoding BioB reduced the bacterial ability to replicate inside the macrophages, suggesting a biotin-limited environment encountered by S. Typhimurium within RAW 264.7 macrophages.

Original languageEnglish (US)
Pages (from-to)388-397
Number of pages10
JournalJournal of Proteomics and Bioinformatics
Volume2
Issue number9
DOIs
StatePublished - Sep 1 2009

Keywords

  • Biotin biosynthesis
  • Low Mg+/pH defined growth medium
  • Salmonella enterica serotype typhimurium
  • Salmonella pathogenicity Island 2
  • Salmonella-containing vacuole
  • Type III secretion system

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Computer Science Applications
  • Cell Biology

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    Shi, L., Ansong, C., Smallwood, H., Rommereim, L., McDermott, J. E., Brewer, H. M., Norbeck, A. D., Taylor, R. C., Gustin, J. K., Heffron, F., Smith, R. D., & Adkins, J. N. (2009). Proteome of Salmonella enterica serotype Typhimurium grown in a low Mg2+/pH medium. Journal of Proteomics and Bioinformatics, 2(9), 388-397. https://doi.org/10.4172/jpb.1000099