The evaluation of protein function in the context of the whole cell is crucial for understanding of living systems. In this context, the identification and modulation of protein-protein interactions in and outside cells is of ample importance. Several methods have been developed in the past years to detect and/or actively induce protein-protein interactions in living cells. As a result, tools are now available to manipulate intracellular events by reversible or irreversible cross-linking of proteins in a specific manner. These techniques open many new doors and enable the dissection of complicated protein networks. Herein we describe which cross-linkers and inducers of dimerization are out there and how to make use of this great toolbox. In pairs: The key to understand living systems is enciphered in protein-protein interaction networks. For unraveling its complexity, a potent toolbox of small molecules (cross-linkers and chemical inducers of dimerization) is available to sense and induce the dynamic interaction processes in living cells.
|Original language||English (US)|
|Number of pages||11|
|Journal||Angewandte Chemie - International Edition|
|State||Published - Aug 13 2012|
- protein-protein interactions
- small molecules
ASJC Scopus subject areas