Protein synthesis and the presence or absence of a measurable G1 in cultured Chinese hamster cells

Robert (Mike) Liskay, B. Kornfeld, P. Fullerton, R. Evans

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

V79-8 cells lack a measurable G1 interval under normal growth conditions. We found that partial inhibition of protein synthesis using low levels of cycloheximide (0.05 μ/ml) could induce a measurable G1 in these cells without any significant effects on S, G2, or M. In view of these findings, recessive mutants selected from the V79-8 cell line, which each express G1, were analyzed for their rates of protein synthesis and degradation/loss. Three of the four mutants showed a decreased rate of protein synthesis sufficent to account for their G1 lengths. A fourth mutant, however, showed parental rates of both protein synthesis and degradation/loss. These results suggest not only that a G1 interval can be expressed as a result of a decreased rate of protein synthesis, but that other alterations (mutations) other than those simply affecting overall protein synthesis can result in the expression of a measurable G1 interval.

Original languageEnglish (US)
Pages (from-to)461-467
Number of pages7
JournalJournal of Cellular Physiology
Volume104
Issue number3
StatePublished - 1980
Externally publishedYes

Fingerprint

Cricetulus
Cells
Proteolysis
Proteins
Cycloheximide
Degradation
Cell Line
Mutation
Growth

ASJC Scopus subject areas

  • Cell Biology
  • Clinical Biochemistry
  • Physiology

Cite this

Protein synthesis and the presence or absence of a measurable G1 in cultured Chinese hamster cells. / Liskay, Robert (Mike); Kornfeld, B.; Fullerton, P.; Evans, R.

In: Journal of Cellular Physiology, Vol. 104, No. 3, 1980, p. 461-467.

Research output: Contribution to journalArticle

Liskay, RM, Kornfeld, B, Fullerton, P & Evans, R 1980, 'Protein synthesis and the presence or absence of a measurable G1 in cultured Chinese hamster cells', Journal of Cellular Physiology, vol. 104, no. 3, pp. 461-467.
Liskay, Robert (Mike) ; Kornfeld, B. ; Fullerton, P. ; Evans, R. / Protein synthesis and the presence or absence of a measurable G1 in cultured Chinese hamster cells. In: Journal of Cellular Physiology. 1980 ; Vol. 104, No. 3. pp. 461-467.
@article{d099500f130f461eb7b0cfb2a3cc86c6,
title = "Protein synthesis and the presence or absence of a measurable G1 in cultured Chinese hamster cells",
abstract = "V79-8 cells lack a measurable G1 interval under normal growth conditions. We found that partial inhibition of protein synthesis using low levels of cycloheximide (0.05 μ/ml) could induce a measurable G1 in these cells without any significant effects on S, G2, or M. In view of these findings, recessive mutants selected from the V79-8 cell line, which each express G1, were analyzed for their rates of protein synthesis and degradation/loss. Three of the four mutants showed a decreased rate of protein synthesis sufficent to account for their G1 lengths. A fourth mutant, however, showed parental rates of both protein synthesis and degradation/loss. These results suggest not only that a G1 interval can be expressed as a result of a decreased rate of protein synthesis, but that other alterations (mutations) other than those simply affecting overall protein synthesis can result in the expression of a measurable G1 interval.",
author = "Liskay, {Robert (Mike)} and B. Kornfeld and P. Fullerton and R. Evans",
year = "1980",
language = "English (US)",
volume = "104",
pages = "461--467",
journal = "Journal of Cellular Physiology",
issn = "0021-9541",
publisher = "Wiley-Liss Inc.",
number = "3",

}

TY - JOUR

T1 - Protein synthesis and the presence or absence of a measurable G1 in cultured Chinese hamster cells

AU - Liskay, Robert (Mike)

AU - Kornfeld, B.

AU - Fullerton, P.

AU - Evans, R.

PY - 1980

Y1 - 1980

N2 - V79-8 cells lack a measurable G1 interval under normal growth conditions. We found that partial inhibition of protein synthesis using low levels of cycloheximide (0.05 μ/ml) could induce a measurable G1 in these cells without any significant effects on S, G2, or M. In view of these findings, recessive mutants selected from the V79-8 cell line, which each express G1, were analyzed for their rates of protein synthesis and degradation/loss. Three of the four mutants showed a decreased rate of protein synthesis sufficent to account for their G1 lengths. A fourth mutant, however, showed parental rates of both protein synthesis and degradation/loss. These results suggest not only that a G1 interval can be expressed as a result of a decreased rate of protein synthesis, but that other alterations (mutations) other than those simply affecting overall protein synthesis can result in the expression of a measurable G1 interval.

AB - V79-8 cells lack a measurable G1 interval under normal growth conditions. We found that partial inhibition of protein synthesis using low levels of cycloheximide (0.05 μ/ml) could induce a measurable G1 in these cells without any significant effects on S, G2, or M. In view of these findings, recessive mutants selected from the V79-8 cell line, which each express G1, were analyzed for their rates of protein synthesis and degradation/loss. Three of the four mutants showed a decreased rate of protein synthesis sufficent to account for their G1 lengths. A fourth mutant, however, showed parental rates of both protein synthesis and degradation/loss. These results suggest not only that a G1 interval can be expressed as a result of a decreased rate of protein synthesis, but that other alterations (mutations) other than those simply affecting overall protein synthesis can result in the expression of a measurable G1 interval.

UR - http://www.scopus.com/inward/record.url?scp=0019254426&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0019254426&partnerID=8YFLogxK

M3 - Article

C2 - 7419616

AN - SCOPUS:0019254426

VL - 104

SP - 461

EP - 467

JO - Journal of Cellular Physiology

JF - Journal of Cellular Physiology

SN - 0021-9541

IS - 3

ER -