Protein phosphorylation in irradiated human melanoma cells

Raymond L. Warters, Dustin L. Williams, Sergey B. Zhuplatov, Chris D. Pond, Sancy Leachman

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

In the present study, we examined the response of confluent, primary human fibroblasts and cells of a melanoma (YUSAC2) cell line to ionizing radiation mediated through post-translational protein phosphorylation. Since the purpose of our study was to identify novel radiation-induced phosphoproteins in the DNA damage stress response of melanoma cells, we were primarily interested in changes in protein phosphoserine expression at early times after irradiation. Our rationale was that by examining the overall protein phosphorylation profile (the phosphoproteome) in irradiated cells, we might discover novel radiation-induced phosphoproteins that distinguish fibroblasts from melanoma cells. Cell proteins were separated by gel electrophoresis and phosphoproteins were identified by Western blot analysis using nonspecific anti-phosphoamino acid antibodies. This approach was not pursued previously since adequate antibodies for examining global protein phosphoserine expression were unavailable. While some radiation-induced phosphoprotein changes in high-abundance proteins were identified, in general the sensitivity of this approach was not sufficient to detect changes in low-abundance, regulatory proteins. Characterization of these phosphoproteins will require greater enrichment of low-abundance proteins.

Original languageEnglish (US)
Pages (from-to)535-544
Number of pages10
JournalRadiation Research
Volume168
Issue number5
DOIs
StatePublished - Nov 2007
Externally publishedYes

Fingerprint

phosphorylation
phosphoproteins
protein phosphorylation
melanoma
Melanoma
Phosphorylation
Phosphoproteins
proteins
cells
Proteins
fibroblasts
Phosphoserine
Radiation
antibodies
regulatory proteins
ionizing radiation
radiation
Fibroblasts
DNA damage
gel electrophoresis

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Radiology Nuclear Medicine and imaging
  • Biophysics
  • Radiation

Cite this

Warters, R. L., Williams, D. L., Zhuplatov, S. B., Pond, C. D., & Leachman, S. (2007). Protein phosphorylation in irradiated human melanoma cells. Radiation Research, 168(5), 535-544. https://doi.org/10.1667/RR0404.1

Protein phosphorylation in irradiated human melanoma cells. / Warters, Raymond L.; Williams, Dustin L.; Zhuplatov, Sergey B.; Pond, Chris D.; Leachman, Sancy.

In: Radiation Research, Vol. 168, No. 5, 11.2007, p. 535-544.

Research output: Contribution to journalArticle

Warters, RL, Williams, DL, Zhuplatov, SB, Pond, CD & Leachman, S 2007, 'Protein phosphorylation in irradiated human melanoma cells', Radiation Research, vol. 168, no. 5, pp. 535-544. https://doi.org/10.1667/RR0404.1
Warters, Raymond L. ; Williams, Dustin L. ; Zhuplatov, Sergey B. ; Pond, Chris D. ; Leachman, Sancy. / Protein phosphorylation in irradiated human melanoma cells. In: Radiation Research. 2007 ; Vol. 168, No. 5. pp. 535-544.
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