TY - JOUR
T1 - Protection against Schistosoma mansoni infection with a recombinant baculovirus-expressed subunit of calpain
AU - Hota-Mitchell, Sheela
AU - Siddiqui, Afzal A.
AU - Dekaban, Gregory A.
AU - Smith, Jana
AU - Tognon, Cristina
AU - Podesta, Ronald B.
N1 - Funding Information:
The authors gratefully acknowledgeD r Michael W. Clarke, Anna Gregoriew, and Dr Jay Keystone for providing the 12 infected patient sera from the Toronto Hospital and Cindy Wong for performing statisticala nalysisof the data. The contributions of Terry Huebert, Mary Keet, Masudul Ansari, and Terry MacGregor for invaluable technical contributions to the CaBP-APM immunizatione xperimentsis acknowledged.T he authorsw ould also like to acknowledgeD r Alan Sher for critical review of the manuscript. Gregory A.. Dekabani s an Ontario Ministry of Health Career Scientist.S heela Hota-Mitchell is supportedb y an Ontario Graduate Student Scholarship. This research was supported by The Medical Research Council of Canada.T he views expressedin this paper are thoseo f the authorsa nd do not reflect thoseo f the Ontario Ministry of Health.
PY - 1997/10
Y1 - 1997/10
N2 - Infections by human schistosomes, in particular Schistosoma mansoni, account for significant morbidity and mortality every year in tropical and sub-tropical areas. The eggs of the parasite induce pathological changes in the infected host; in chronic and heavy infections, these changes may lead to death. A well-designed anti-schistosomal vaccine, alone or in concert with existing control measures such as chemotherapy, may prove to be a safe, inexpensive and effective means of reducing the occurrence of severe disease and death in S. mansoni infection. Previous studies have demonstrated the importance of the syncytial layer containing the apical plasma membrane (APM) of S. mansoni in both the survival of the parasite in the mammalian host and as a potential source of immunogens which may be utilized as vaccine candidates. In this paper we present evidence for the protective capacity of several schistosomal antigen preparations, including a calcium binding protein of the APM, S. mansoni calpain (GenBnnk accession no. M74233). We have constructed and characterized expression of a recombinant baculovirus expressing the large subunit of S. mansoni calpain, Sm-p80. This recombinant Sm-p80 is recognized by IgA, IgM, IgG1, and IgG3 isotype antibodies found in S. mansoni-infected human sera and partially-purified recombinant Sm-p80 provided a 29-39% reduction in worm burden in immunized mice challenged with S. mansoni. Our data indicate that Sm-p80 may be a useful vaccine antigen for the reduction of the morbidity associated with S. mansoni infections of mammalian hosts.
AB - Infections by human schistosomes, in particular Schistosoma mansoni, account for significant morbidity and mortality every year in tropical and sub-tropical areas. The eggs of the parasite induce pathological changes in the infected host; in chronic and heavy infections, these changes may lead to death. A well-designed anti-schistosomal vaccine, alone or in concert with existing control measures such as chemotherapy, may prove to be a safe, inexpensive and effective means of reducing the occurrence of severe disease and death in S. mansoni infection. Previous studies have demonstrated the importance of the syncytial layer containing the apical plasma membrane (APM) of S. mansoni in both the survival of the parasite in the mammalian host and as a potential source of immunogens which may be utilized as vaccine candidates. In this paper we present evidence for the protective capacity of several schistosomal antigen preparations, including a calcium binding protein of the APM, S. mansoni calpain (GenBnnk accession no. M74233). We have constructed and characterized expression of a recombinant baculovirus expressing the large subunit of S. mansoni calpain, Sm-p80. This recombinant Sm-p80 is recognized by IgA, IgM, IgG1, and IgG3 isotype antibodies found in S. mansoni-infected human sera and partially-purified recombinant Sm-p80 provided a 29-39% reduction in worm burden in immunized mice challenged with S. mansoni. Our data indicate that Sm-p80 may be a useful vaccine antigen for the reduction of the morbidity associated with S. mansoni infections of mammalian hosts.
KW - Calpain
KW - Recombinant baculovirus expression system
KW - Schistosoma mansoni
KW - Vaccine
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U2 - 10.1016/S0264-410X(97)00081-9
DO - 10.1016/S0264-410X(97)00081-9
M3 - Article
C2 - 9364694
AN - SCOPUS:0030873953
SN - 0264-410X
VL - 15
SP - 1631
EP - 1640
JO - Vaccine
JF - Vaccine
IS - 15
ER -