Prostaglandin-endoperoxide synthase (PTGS1 and PTGS2) expression and prostaglandin production by normal monkey ovarian surface epithelium

Rafael A. Cabrera, Brandy Dozier, Diane M. Duffy

Research output: Contribution to journalArticle

Abstract

Objective: To determine whether hCG regulates the expression of prostaglandin (PG) synthesis enzymes and the production of PGs by normal monkey ovarian surface epithelium (OSE). Design: Experimental animal study. Setting: Research laboratory. Animal(s): Adult cynomolgus macaques. Intervention(s): Monkeys received exogenous gonadotropins to stimulate multiple follicular development. Ovarian surface epithelium cells and whole ovaries were obtained before (0 hours) and 36 hours after an ovulatory dose of hCG. Main Outcome Measure(s): Ovarian surface epithelium expression of prostaglandin-endoperoxide synthase 1 (PTGS1) and PTGS2 proteins was determined by immunocytochemistry. Prostaglandin synthesis enzyme messenger RNA (mRNA) levels were determined by RT-PCR. Prostaglandin E2 and PGF production was assessed by enzyme immunoassays. Result(s): Ovarian surface epithelium maintained in long-term culture expressed mRNA and protein for PTGS1 and PTGS2 (n = 6); inhibition of PTGS1, but not PTGS2, reduced PGE2 synthesis (n = 3). Prostaglandin-endoperoxide synthase 1 was present in OSE of ovarian tissue sections obtained 0 (n = 4) and 36 (n = 3) hours after hCG; PTGS2 was not detected. Ovarian surface epithelium collected 0 (n = 3) and 36 (n = 4) hours after hCG expressed mRNAs for PTGS1, PTGS2, and three PGE synthases; the ratio of PTGS2 to PTGS1 increased in response to hCG exposure. Conclusion(s): Monkey OSE expresses mRNA for PTGS1, PTGS2, and all PGE synthases and produces PGE2 both before and 36 hours after hCG. Detection of PTGS1, but not PTGS2, protein in OSE in vivo supports the hypothesis that PTGS1 is the enzyme responsible for PGE2 production by primate OSE in vivo.

Original languageEnglish (US)
Pages (from-to)1088-1096
Number of pages9
JournalFertility and Sterility
Volume86
Issue number4 SUPPL.
DOIs
StatePublished - Oct 2006
Externally publishedYes

Fingerprint

Cyclooxygenase 2
Prostaglandin-Endoperoxide Synthases
Prostaglandins
Haplorhini
Epithelium
Dinoprostone
Messenger RNA
Prostaglandins E
Enzymes
Proteins
Dinoprost
Macaca
Gonadotropins
Immunoenzyme Techniques
Primates
Ovary
Research Design
Immunohistochemistry
Outcome Assessment (Health Care)
Polymerase Chain Reaction

Keywords

  • cyclooxygenase
  • ovarian cancer
  • ovary
  • ovulation
  • primate
  • Prostaglandin

ASJC Scopus subject areas

  • Obstetrics and Gynecology

Cite this

Prostaglandin-endoperoxide synthase (PTGS1 and PTGS2) expression and prostaglandin production by normal monkey ovarian surface epithelium. / Cabrera, Rafael A.; Dozier, Brandy; Duffy, Diane M.

In: Fertility and Sterility, Vol. 86, No. 4 SUPPL., 10.2006, p. 1088-1096.

Research output: Contribution to journalArticle

@article{745b31da70f1401aaa30deebacd660d0,
title = "Prostaglandin-endoperoxide synthase (PTGS1 and PTGS2) expression and prostaglandin production by normal monkey ovarian surface epithelium",
abstract = "Objective: To determine whether hCG regulates the expression of prostaglandin (PG) synthesis enzymes and the production of PGs by normal monkey ovarian surface epithelium (OSE). Design: Experimental animal study. Setting: Research laboratory. Animal(s): Adult cynomolgus macaques. Intervention(s): Monkeys received exogenous gonadotropins to stimulate multiple follicular development. Ovarian surface epithelium cells and whole ovaries were obtained before (0 hours) and 36 hours after an ovulatory dose of hCG. Main Outcome Measure(s): Ovarian surface epithelium expression of prostaglandin-endoperoxide synthase 1 (PTGS1) and PTGS2 proteins was determined by immunocytochemistry. Prostaglandin synthesis enzyme messenger RNA (mRNA) levels were determined by RT-PCR. Prostaglandin E2 and PGF2α production was assessed by enzyme immunoassays. Result(s): Ovarian surface epithelium maintained in long-term culture expressed mRNA and protein for PTGS1 and PTGS2 (n = 6); inhibition of PTGS1, but not PTGS2, reduced PGE2 synthesis (n = 3). Prostaglandin-endoperoxide synthase 1 was present in OSE of ovarian tissue sections obtained 0 (n = 4) and 36 (n = 3) hours after hCG; PTGS2 was not detected. Ovarian surface epithelium collected 0 (n = 3) and 36 (n = 4) hours after hCG expressed mRNAs for PTGS1, PTGS2, and three PGE synthases; the ratio of PTGS2 to PTGS1 increased in response to hCG exposure. Conclusion(s): Monkey OSE expresses mRNA for PTGS1, PTGS2, and all PGE synthases and produces PGE2 both before and 36 hours after hCG. Detection of PTGS1, but not PTGS2, protein in OSE in vivo supports the hypothesis that PTGS1 is the enzyme responsible for PGE2 production by primate OSE in vivo.",
keywords = "cyclooxygenase, ovarian cancer, ovary, ovulation, primate, Prostaglandin",
author = "Cabrera, {Rafael A.} and Brandy Dozier and Duffy, {Diane M.}",
year = "2006",
month = "10",
doi = "10.1016/j.fertnstert.2006.03.022",
language = "English (US)",
volume = "86",
pages = "1088--1096",
journal = "Fertility and Sterility",
issn = "0015-0282",
publisher = "Elsevier Inc.",
number = "4 SUPPL.",

}

TY - JOUR

T1 - Prostaglandin-endoperoxide synthase (PTGS1 and PTGS2) expression and prostaglandin production by normal monkey ovarian surface epithelium

AU - Cabrera, Rafael A.

AU - Dozier, Brandy

AU - Duffy, Diane M.

PY - 2006/10

Y1 - 2006/10

N2 - Objective: To determine whether hCG regulates the expression of prostaglandin (PG) synthesis enzymes and the production of PGs by normal monkey ovarian surface epithelium (OSE). Design: Experimental animal study. Setting: Research laboratory. Animal(s): Adult cynomolgus macaques. Intervention(s): Monkeys received exogenous gonadotropins to stimulate multiple follicular development. Ovarian surface epithelium cells and whole ovaries were obtained before (0 hours) and 36 hours after an ovulatory dose of hCG. Main Outcome Measure(s): Ovarian surface epithelium expression of prostaglandin-endoperoxide synthase 1 (PTGS1) and PTGS2 proteins was determined by immunocytochemistry. Prostaglandin synthesis enzyme messenger RNA (mRNA) levels were determined by RT-PCR. Prostaglandin E2 and PGF2α production was assessed by enzyme immunoassays. Result(s): Ovarian surface epithelium maintained in long-term culture expressed mRNA and protein for PTGS1 and PTGS2 (n = 6); inhibition of PTGS1, but not PTGS2, reduced PGE2 synthesis (n = 3). Prostaglandin-endoperoxide synthase 1 was present in OSE of ovarian tissue sections obtained 0 (n = 4) and 36 (n = 3) hours after hCG; PTGS2 was not detected. Ovarian surface epithelium collected 0 (n = 3) and 36 (n = 4) hours after hCG expressed mRNAs for PTGS1, PTGS2, and three PGE synthases; the ratio of PTGS2 to PTGS1 increased in response to hCG exposure. Conclusion(s): Monkey OSE expresses mRNA for PTGS1, PTGS2, and all PGE synthases and produces PGE2 both before and 36 hours after hCG. Detection of PTGS1, but not PTGS2, protein in OSE in vivo supports the hypothesis that PTGS1 is the enzyme responsible for PGE2 production by primate OSE in vivo.

AB - Objective: To determine whether hCG regulates the expression of prostaglandin (PG) synthesis enzymes and the production of PGs by normal monkey ovarian surface epithelium (OSE). Design: Experimental animal study. Setting: Research laboratory. Animal(s): Adult cynomolgus macaques. Intervention(s): Monkeys received exogenous gonadotropins to stimulate multiple follicular development. Ovarian surface epithelium cells and whole ovaries were obtained before (0 hours) and 36 hours after an ovulatory dose of hCG. Main Outcome Measure(s): Ovarian surface epithelium expression of prostaglandin-endoperoxide synthase 1 (PTGS1) and PTGS2 proteins was determined by immunocytochemistry. Prostaglandin synthesis enzyme messenger RNA (mRNA) levels were determined by RT-PCR. Prostaglandin E2 and PGF2α production was assessed by enzyme immunoassays. Result(s): Ovarian surface epithelium maintained in long-term culture expressed mRNA and protein for PTGS1 and PTGS2 (n = 6); inhibition of PTGS1, but not PTGS2, reduced PGE2 synthesis (n = 3). Prostaglandin-endoperoxide synthase 1 was present in OSE of ovarian tissue sections obtained 0 (n = 4) and 36 (n = 3) hours after hCG; PTGS2 was not detected. Ovarian surface epithelium collected 0 (n = 3) and 36 (n = 4) hours after hCG expressed mRNAs for PTGS1, PTGS2, and three PGE synthases; the ratio of PTGS2 to PTGS1 increased in response to hCG exposure. Conclusion(s): Monkey OSE expresses mRNA for PTGS1, PTGS2, and all PGE synthases and produces PGE2 both before and 36 hours after hCG. Detection of PTGS1, but not PTGS2, protein in OSE in vivo supports the hypothesis that PTGS1 is the enzyme responsible for PGE2 production by primate OSE in vivo.

KW - cyclooxygenase

KW - ovarian cancer

KW - ovary

KW - ovulation

KW - primate

KW - Prostaglandin

UR - http://www.scopus.com/inward/record.url?scp=33748890479&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33748890479&partnerID=8YFLogxK

U2 - 10.1016/j.fertnstert.2006.03.022

DO - 10.1016/j.fertnstert.2006.03.022

M3 - Article

C2 - 16962117

AN - SCOPUS:33748890479

VL - 86

SP - 1088

EP - 1096

JO - Fertility and Sterility

JF - Fertility and Sterility

SN - 0015-0282

IS - 4 SUPPL.

ER -