TY - JOUR
T1 - Prosomatostatin-specific antigen in rat brain
T2 - Localization by immunocytochemical staining with an antiserum to a synthetic sequence of preprosomatostatin
AU - Lechan, R. M.
AU - Goodman, R. H.
AU - Rosenblatt, M.
AU - Reichlin, S.
AU - Habener, J. F.
PY - 1983
Y1 - 1983
N2 - Using an antiserum to a 15-amino acid synthetic peptide corresponding to amino acids 63-77 of rat preprosomatostatin (rat somatostatin cryptic peptide, RSCP), we have compared the distribution of immunoreactive RSCP (IR-RSCP) with that of immunoreactive somatostatin-14 in the rat brain. IR-RSCP was present in neuronal cell bodies, processes, and axon terminals in the hypothalamic tuberoinfundibular system as well as in diverse regions of the central nervous system in an identical distribution to immunoreactive somatostatin. These observations indicate that in neurons the somatostatin prohormone or the NH2-terminal extension peptide of somatostatin-28 (or both) is stored and transported intracellularly along with somatostatin 14. In addition, the presence of IR-RSCP in nerve terminals suggests that this material may be secreted as a hormone or neuromodulator and may serve as a biologic marker of somatostatin secretion.
AB - Using an antiserum to a 15-amino acid synthetic peptide corresponding to amino acids 63-77 of rat preprosomatostatin (rat somatostatin cryptic peptide, RSCP), we have compared the distribution of immunoreactive RSCP (IR-RSCP) with that of immunoreactive somatostatin-14 in the rat brain. IR-RSCP was present in neuronal cell bodies, processes, and axon terminals in the hypothalamic tuberoinfundibular system as well as in diverse regions of the central nervous system in an identical distribution to immunoreactive somatostatin. These observations indicate that in neurons the somatostatin prohormone or the NH2-terminal extension peptide of somatostatin-28 (or both) is stored and transported intracellularly along with somatostatin 14. In addition, the presence of IR-RSCP in nerve terminals suggests that this material may be secreted as a hormone or neuromodulator and may serve as a biologic marker of somatostatin secretion.
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U2 - 10.1073/pnas.80.9.2780
DO - 10.1073/pnas.80.9.2780
M3 - Article
C2 - 6133283
AN - SCOPUS:0020540675
VL - 80
SP - 2780
EP - 2784
JO - Indian Journal of Rheumatology
JF - Indian Journal of Rheumatology
SN - 0973-3698
IS - 9 I
ER -